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AB231

Anti-EGFR 抗体 [ICR10]

Anti-EGFR antibody [ICR10]

4

(7 Reviews)

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(33 Publications)

Anti-EGFR antibody [ICR10] (ab231) is a rat monoclonal antibody detecting EGFR in Flow Cytometry, ICC/IF. Suitable for Human.

- Over 30 publications
- Trusted since 1999

查看别名

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

2 Images
Flow Cytometry - Anti-EGFR antibody [ICR10] (AB231)
  • Flow Cyt

Lab

Flow Cytometry - Anti-EGFR antibody [ICR10] (AB231)

Flow cytometric analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labelling EGFR with ab231 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) / Blue.

Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 647, ab150163) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.

Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [ICR10] (AB231)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [ICR10] (AB231)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling EGFR with ab231 at 1/50 dilution followed by secondary antibody ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (green).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used as a counterstain at a 1/200 dilution followed by a secondary antibody ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) used at a 1/1000 dilution (red).

Confocal image showing strong membranous and weak cytoplasmic staining in A431 cells.
Low expression control : HEK-293 (PMID : 26368334).

Nuclear DNA was labelled with DAPI (shown in blue).

不同偶联物与剂型 (2)

  • 578 PE

    PE Anti-EGFR antibody [ICR10]

  • 519 FITC

    FITC Anti-EGFR antibody [ICR10]

关键信息

宿主种属

Rat

克隆

Monoclonal

克隆号

ICR10

亚型

IgG2a

轻链类型

unknown

不含载体蛋白

No

反应种属

Human

应用

ICC/IF, Flow Cyt

applications

免疫原

Native Full Length Protein corresponding to Human EGFR.

P00533

表位

ICR10 binds to epitope B, and has an affinity of 6.7x10-9 M.

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反应性数据

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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

What is this antibody validated in?
Anti-EGFR antibody [ICR10] (ab231) is a rat monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trusted by the scientific community
Anti-EGFR [ICR10] (ab231) was first used in a scientific publication in 1999 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-EGFR [ICR10] (ab231) has over 5 independent reviews from customers.

Other related products
We have a range of other formats of antibody clone [ICR10] also available for your convenience: ab231, FITC - ab11400, PE - ab27764

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Ion exchange chromatography
纯化说明
Purified IgG prepared by ion exchange chromatography.
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

The protein expressed by the EGFR gene functions as a receptor tyrosine kinase, binding with ligands from the EGF family to activate several signaling cascades that translate extracellular signals into cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen, BTC/betacellulin, epiregulin, and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation, recruiting adapter proteins like GRB2 and activating complex downstream signaling pathways such as RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STATs, and possibly the NF-kappa-B cascade. EGFR also directly phosphorylates proteins like RGS16 and MUC1, influencing various cellular processes including its coupling with G protein-coupled receptor signaling and cell migration through interaction with CCDC88A/GIV. Isoform 2 of EGFR may act as an antagonist to EGF actions. In microbial infection, EGFR also serves as a receptor facilitating hepatitis C virus (HCV) entry into hepatocytes by promoting CD81-CLDN1 receptor complexes and enhancing membrane fusion with HCV envelope glycoproteins. This supplementary information is collated from multiple sources and compiled automatically.
See full target information EGFR
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文献 (33)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed36831214

2023

Dual Targeting of EGFR and MTOR Pathways Inhibits Glioblastoma Growth by Modulating the Tumor Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Maxim Sidorov,Pratiksha Dighe,Rinette W L Woo,Aida Rodriguez-Brotons,Michelle Chen,Ryan J Ice,Edith Vaquero,Damon Jian,Pierre-Yves Desprez,Mehdi Nosrati,Leah Galvez,Lewis Leng,Lawrence Dickinson,Mohammed Kashani-Sabet,Sean David McAllister,Liliana Soroceanu

STAR protocols 3:101475 PubMed35755125

2022

Measuring EGFR plasma membrane density, stability, internalization, and recycling in alive adherent cells by cell surface ELISA.

Applications

Unspecified application

Species

Unspecified reactive species

Jalal M Kazan,Ariadna Pollato-Blanco,Gergely L Lukacs,Arnim Pause

IEEE open journal of engineering in medicine and biology 3:86-95 PubMed35813488

2022

Assessment of CAR-T Cell-Mediated Cytotoxicity in 3D Microfluidic Cancer Co-Culture Models for Combination Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Karla Paterson,Sarah Paterson,Theresa Mulholland,Seth B Coffelt,Michele Zagnoni

Nature cancer 3:418-436 PubMed35469014

2022

Functional patient-derived organoid screenings identify MCLA-158 as a therapeutic EGFR × LGR5 bispecific antibody with efficacy in epithelial tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Bram Herpers,Berina Eppink,Mark I James,Carme Cortina,Adrià Cañellas-Socias,Sylvia F Boj,Xavier Hernando-Momblona,Dominik Glodzik,Rob C Roovers,Marc van de Wetering,Carina Bartelink-Clements,Vanessa Zondag-van der Zande,Jara García Mateos,Kuan Yan,Lucia Salinaro,Abdul Basmeleh,Szabolcs Fatrai,David Maussang,Jeroen J Lammerts van Bueren,Irene Chicote,Garazi Serna,Laia Cabellos,Lorena Ramírez,Paolo Nuciforo,Ramon Salazar,Cristina Santos,Alberto Villanueva,Camille Stephan-Otto Attolini,Elena Sancho,Hector G Palmer,Josep Tabernero,Michael R Stratton,John de Kruif,Ton Logtenberg,Hans Clevers,Leo S Price,Robert G J Vries,Eduard Batlle,Mark Throsby

International journal of molecular sciences 22: PubMed34948279

2021

Two-Chains Tissue Plasminogen Activator Unifies Met and NMDA Receptor Signalling to Control Neuronal Survival.

Applications

Unspecified application

Species

Unspecified reactive species

Elodie Hedou,Sara Douceau,Arnaud Chevilley,Alexandre Varangot,Audrey M Thiebaut,Hortense Triniac,Isabelle Bardou,Carine Ali,Mike Maillasson,Tiziana Crepaldi,Paolo Comoglio,Eloïse Lemarchand,Véronique Agin,Benoit D Roussel,Denis Vivien

iScience 24:103274 PubMed34761192

2021

Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes.

Applications

Unspecified application

Species

Unspecified reactive species

Jalal M Kazan,Guillaume Desrochers,Claire E Martin,Hyeonju Jeong,Dmitri Kharitidi,Pirjo M Apaja,Ariel Roldan,Nicole St Denis,Anne-Claude Gingras,Gergely L Lukacs,Arnim Pause

Cell 184:5053-5069.e23 PubMed34390642

2021

Chromatin and gene-regulatory dynamics of the developing human cerebral cortex at single-cell resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandro E Trevino,Fabian Müller,Jimena Andersen,Laksshman Sundaram,Arwa Kathiria,Anna Shcherbina,Kyle Farh,Howard Y Chang,Anca M Pașca,Anshul Kundaje,Sergiu P Pașca,William J Greenleaf

Cell reports 34:108788 PubMed33657375

2021

Heterogeneity of glial progenitor cells during the neurogenesis-to-gliogenesis switch in the developing human cerebral cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Fu,Ming Yang,Hongmin Yu,Yicheng Wang,Xinglong Wu,Jun Yong,Yunuo Mao,Yueli Cui,Xiaoying Fan,Lu Wen,Jie Qiao,Fuchou Tang

Cell reports 31:107599 PubMed32375049

2020

Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates.

Applications

Unspecified application

Species

Unspecified reactive species

Nicola Micali,Suel-Kee Kim,Marcelo Diaz-Bustamante,Genevieve Stein-O'Brien,Seungmae Seo,Joo-Heon Shin,Brian G Rash,Shaojie Ma,Yanhong Wang,Nicolas A Olivares,Jon I Arellano,Kristen R Maynard,Elana J Fertig,Alan J Cross,Roland W Bürli,Nicholas J Brandon,Daniel R Weinberger,Joshua G Chenoweth,Daniel J Hoeppner,Nenad Sestan,Pasko Rakic,Carlo Colantuoni,Ronald D McKay

Scientific reports 9:14611 PubMed31601953

2019

CD206 tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production.

Applications

Unspecified application

Species

Unspecified reactive species

A S M Rafiul Haque,Masafumi Moriyama,Keigo Kubota,Noriko Ishiguro,Mizuki Sakamoto,Akira Chinju,Keita Mochizuki,Taiki Sakamoto,Naoki Kaneko,Ryusuke Munemura,Takashi Maehara,Akihiko Tanaka,Jun-Nosuke Hayashida,Shintaro Kawano,Tamotsu Kiyoshima,Seiji Nakamura
View all publications
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我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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