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AB76153

重组Anti-EGFR抗体[EPR39Y]

Anti-EGFR antibody [EPR39Y]

  • RabMAb
  • Recombinant
  • Lab Essentials
  • 20ul selling size
  • 了解详情

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(6 Publications)

Rabbit Recombinant Monoclonal EGFR antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 6 publications.

查看别名

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EPR39Y] (AB76153)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EPR39Y] (AB76153)

ab76153 at 1/100 dilution staining EGFR in A431 cells by Immunofluorescence.

Flow Cytometry (Intracellular) - Anti-EGFR antibody [EPR39Y] (AB76153)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-EGFR antibody [EPR39Y] (AB76153)

Overlay histogram showing A431 cells stained with ab76153 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76153, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Western blot - Anti-EGFR antibody [EPR39Y] (AB76153)
  • WB

Unknown

Western blot - Anti-EGFR antibody [EPR39Y] (AB76153)

All lanes:

Western blot - Anti-EGFR antibody [EPR39Y] (ab76153) at 1/5000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/1000 dilution

Predicted band size: 134 kDa

Observed band size: 170 kDa,180 kDa

true

Western blot - Anti-EGFR antibody [EPR39Y] (AB76153)
  • WB

Lab

Western blot - Anti-EGFR antibody [EPR39Y] (AB76153)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab76153 overnight at 4°C in the presence of loading control ab18058 (Mouse monoclonal [SPM227] to Vinculin diluted 1 : 10000). Antibody binding was detected using IR-labelled goat anti-Rabbit Ab at a 1 : 10,000 dilution for one hour at room temperature before imaging.

This product yielded a strong signal in western blot using A431 (human squamous carcinoma) lysate which naturally overexpresses the EGFR protein. Western blot conditions may need to be optimised for cell lines and tissues that express lower levels of endogenous EGFR.

All lanes:

Western blot - Anti-EGFR antibody [EPR39Y] (ab76153) at 1/1000 dilution

Lane 1:

Caco-2 cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

Mouse skin cell lysate at 20 µg

Predicted band size: 134 kDa

false

不同偶联物与剂型 (2)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR39Y

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

Flow Cyt (Intra), ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody detects both phosphorylated and non-phosphorylated EGFR protein. This product yielded a strong signal in western blot using A431 (human squamous carcinoma) lysate which naturally overexpresses the EGFR protein. Western blot conditions may need to be optimised for cell lines and tissues that express lower levels of endogenous EGFR.

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反应性数据

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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed : 10805725, PubMed : 27153536, PubMed : 2790960, PubMed : 35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed : 12297049, PubMed : 15611079, PubMed : 17909029, PubMed : 20837704, PubMed : 27153536, PubMed : 2790960, PubMed : 7679104, PubMed : 8144591, PubMed : 9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed : 27153536). May also activate the NF-kappa-B signaling cascade (PubMed : 11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed : 11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed : 11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed : 20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity).. Isoform 2 may act as an antagonist of EGF action.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.
See full target information EGFR
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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Small (Weinheim an der Bergstrasse, Germany) 18:e2204485 PubMed36207287

2022

3D-Engineered Scaffolds to Study Microtubes and Localization of Epidermal Growth Factor Receptor in Patient-Derived Glioma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nastaran Barin,Hayri E Balcioglu,Iris de Heer,Maurice de Wit,Martine L M Lamfers,Martin E van Royen,Pim J French,Angelo Accardo

Journal of cellular biochemistry 122:1277-1294 PubMed34037277

2021

SENP1 participates in Irinotecan resistance in human colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Cheng Chen,Do Chi Nhan,Chiung-Hung Hsu,Tso-Fu Wang,Chi-Cheng Li,Tsung-Jung Ho,B Mahalakshmi,Mei-Chih Chen,Liang-Yo Yang,Chih-Yang Huang

Autophagy 16:435-450 PubMed31203721

2019

Inactivation of MTOR promotes autophagy-mediated epithelial injury in particulate matter-induced airway inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Yin-Fang Wu,Zhou-Yang Li,Ling-Ling Dong,Wei-Jie Li,Yan-Ping Wu,Jing Wang,Hai-Pin Chen,Hui-Wen Liu,Miao Li,Ci-Liang Jin,Hua-Qiong Huang,Song-Min Ying,Wen Li,Hua-Hao Shen,Zhi-Hua Chen

PloS one 13:e0193170 PubMed29494634

2018

Vitamin C alters the amount of specific endoplasmic reticulum associated proteins involved in lipid metabolism in the liver of mice synthesizing a nonfunctional Werner syndrome (Wrn) mutant protein.

Applications

Unspecified application

Species

Unspecified reactive species

Lucie Aumailley,Florence Roux-Dalvai,Isabelle Kelly,Arnaud Droit,Michel Lebel

Scientific reports 7:14222 PubMed29079814

2017

The role of Thyroid Transcription Factor-1 and Tumor differentiation in Resected Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tsai-Wang Huang,Ke- Feng Lin,Chien-Hsing Lee,Hung Chang,Shih-Chun Lee,Yi-Shing Shieh

Oncology letters 10:1495-1500 PubMed26622697

2015

RNA interference for epidermal growth factor receptor enhances the radiosensitivity of esophageal squamous cell carcinoma cell line Eca109.

Applications

WB

Species

Human

Heping Zhang,Jiancheng Li,Wenfang Cheng,D I Liu,Cheng Chen,Xiaoying Wang,Xujing Lu,Xifa Zhou
View all publications
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