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AB270342

重组Anti-EGFR抗体[EPR22309-151] - BSA and Azide free (Capture/Detector)

Anti-EGFR antibody [EPR22309-151] - BSA and Azide free (Capture/Detector)

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Rabbit Recombinant Monoclonal EGFR antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

3 Images
Sandwich ELISA - Anti-EGFR antibody [EPR22309-151] - BSA and Azide free (Capture/Detector) (AB270342)
  • sELISA

Lab

Sandwich ELISA - Anti-EGFR antibody [EPR22309-151] - BSA and Azide free (Capture/Detector) (AB270342)

HeLa (starved) + EGF : HeLa starved overnight, then treated with 100 ng/mL EGF for 30 minutes, whole cell lysate. Serial dilutions of lysates were analyzed. EGFR KO HeLa (starved) + EGF : EGFR knockout HeLa starved overnight, then treated with 100 ng/mL EGF for 30 minutes, whole cell lysate. Serial dilutions of lysates were analyzed.

Sandwich ELISA - Anti-EGFR antibody [EPR22309-151] - BSA and Azide free (Capture/Detector) (AB270342)
  • sELISA

Lab

Sandwich ELISA - Anti-EGFR antibody [EPR22309-151] - BSA and Azide free (Capture/Detector) (AB270342)

HeLa (starved) + EGF : HeLa starved overnight, then treated with 100 ng/mL EGF for 30 minutes, whole cell lysate. Serial dilutions of lysates were analyzed. HeLa (starved) Control : HeLa starved overnight, whole cell lysate. Serial dilutions of lysates were analyzed.

Sandwich ELISA - Anti-EGFR antibody [EPR22309-151] - BSA and Azide free (Capture/Detector) (AB270342)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-EGFR antibody [EPR22309-151] - BSA and Azide free (Capture/Detector) (AB270342)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab269558).

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22309-151

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Assay Kits

AB269558

Human soluble EGFR ELISA Kit

Recombinant|SimpleStep

ELISA - Human soluble EGFR ELISA Kit (AB269558)

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Sample Prep & Detection Kits

AB102899

PE/Cy5.5® Conjugation Kit - Lightning-Link®

Flow Cytometry - PE/Cy5.5® Conjugation Kit - Lightning-Link® (AB102899)

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Sample Prep & Detection Kits

AB269889

Europium Conjugation Kit

Dot Blot - Europium Conjugation Kit (AB269889)

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Primary Antibodies

AB285053

Anti-IL-13 antibody [EPR20465-131] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL-13 antibody [EPR20465-131] - BSA and Azide free (Capture) (AB285053)

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Primary Antibodies

AB269393

Anti-CD200 / OX2 antibody [EPR22412-54] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CD200 / OX2 antibody [EPR22412-54] - BSA and Azide free (Detector) (AB269393)

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Primary Antibodies

AB244843

Anti-IL-31 antibody [EPR20205-211] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL-31 antibody [EPR20205-211] - BSA and Azide free (Capture) (AB244843)

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Primary Antibodies

AB259450

Anti-Midkine antibody [EPR1143-68R] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Midkine antibody [EPR1143-68R] - BSA and Azide free (Detector) (AB259450)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab270342 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human soluble EGFR. The recommended pair for sandwich ELISA is:
Capture: ab270342, Human soluble EGFR Capture Antibody (unconjugated)
Detector: ab270343, Human soluble EGFR Detector Antibody (unconjugated)
The reference range value is 109.375 - 7000 ng/ml.

ab270342 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human EGFR (phospho Y1197). The recommended pair for sandwich ELISA is:

Capture: ab325705, Human EGFR (phospho Y1197) Capture Antibody (unconjugated)

Detector: ab270342, Human EGFR (phospho Y1197) Detector Antibody (unconjugated)

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed : 10805725, PubMed : 27153536, PubMed : 2790960, PubMed : 35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed : 12297049, PubMed : 15611079, PubMed : 17909029, PubMed : 20837704, PubMed : 27153536, PubMed : 2790960, PubMed : 7679104, PubMed : 8144591, PubMed : 9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed : 27153536). May also activate the NF-kappa-B signaling cascade (PubMed : 11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed : 11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed : 11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed : 20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity).. Isoform 2 may act as an antagonist of EGF action.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.
See full target information EGFR
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