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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases

Anti-EGFR抗体[EGFR1] (ab30)

  • Datasheet
  • SDS
Reviews (4)Q&A (4)References (32)

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Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EGFR1] (ab30)
  • Flow Cytometry - Anti-EGFR antibody [EGFR1] (ab30)
  • Immunohistochemistry (Frozen sections) - Anti-EGFR antibody [EGFR1] (ab30)

Key features and details

  • Mouse monoclonal [EGFR1] to EGFR
  • Suitable for: IHC-Fr, ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2b

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概述

  • 产品名称

    Anti-EGFR抗体[EGFR1]
    参阅全部 EGFR 一抗
  • 描述

    小鼠单克隆抗体[EGFR1] to EGFR
  • 宿主

    Mouse
  • 特异性

    Recognises the external EGF-binding domain of the EGFR transmembrane glycoprotein. No effect on tyrosine kinase activity of EGFR.

  • 经测试应用

    适用于: IHC-Fr, ICC/IF, Flow Cytmore details
    不适用于: ELISA or WB
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Horse
  • 免疫原

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • 表位

    Extracellular epitope mapped between aa 6-273 of human EGFR.
  • 阳性对照

    • IHC-Fr: Frozen normal human placenta ICC/IF: A431 cells. Flow Cyt: A431 cells
  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    EGFR1
  • 骨髓瘤

    P3-NS1/1-Ag4-1
  • 同种型

    IgG2b
  • 轻链类型

    kappa
  • 研究领域

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • EGF
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Growth factors
    • EGF
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • Receptor tyrosine kinases
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Cancer
    • Tumor biomarkers
    • Receptors
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

相关产品

  • Alternative Versions

    • PE Anti-EGFR antibody [EGFR1] (ab130738)
    • PE/Cy7® Anti-EGFR antibody [EGFR1] (ab239309)
    • Anti-EGFR antibody [EGFR1] - BSA and Azide free (ab264523)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant human EGFR protein (Active) (ab155639)
    • Recombinant human EGFR protein (Fc Chimera) (ab84002)
  • Related Products

    • Human EGFR ELISA Kit (ERBB) (ab100505)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab30于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
Use a concentration of 10 µg/ml.
ICC/IF (1)
Use a concentration of 1 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

说明
IHC-Fr
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 1 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

应用说明
Is unsuitable for ELISA or WB.

靶标

  • 功能

    Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • 组织特异性

    Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • 疾病相关

    Lung cancer
    Inflammatory skin and bowel disease, neonatal, 2
  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰

    Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
    Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
  • 细胞定位

    Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
  • Target information above from: UniProt accession P00533 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1956 Human
    • Entrez Gene: 13649 Mouse
    • Omim: 131550 Human
    • SwissProt: P00533 Human
    • SwissProt: Q01279 Mouse
    • Unigene: 488293 Human
    • Unigene: 605083 Human
    • Unigene: 420648 Mouse
    • Unigene: 439882 Mouse
    • Unigene: 8534 Mouse
    see all
  • 别名

    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EGFR1] (ab30)
    Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EGFR1] (ab30)

    ab30 staining EGFR in A431 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab30 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Flow Cytometry - Anti-EGFR antibody [EGFR1] (ab30)
    Flow Cytometry - Anti-EGFR antibody [EGFR1] (ab30)

    Overlay histograms showing left positive A431 cells and right negative Jurkat cells stained with ab30 (red line). The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab30, 1x106 cells in 100ul at 1ug/ml) for 30 min on ice. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) preabsorbed (ab150117) at 1/2000 dilution for 30 min on ice.

    Isotype control antibody (black line) was mouse IgG2bκ (ab170192) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  • Immunohistochemistry (Frozen sections) - Anti-EGFR antibody [EGFR1] (ab30)
    Immunohistochemistry (Frozen sections) - Anti-EGFR antibody [EGFR1] (ab30)

    IHC image of EGFR staining in a section of frozen normal human placenta, fixed in 10% paraformaldehyde (10 min). Staining was performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab30, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (32)

发表研究结果有使用 ab30?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab30 被引用在 32 文献中.

  • Lima Moura S  et al. Matrix Effect in the Isolation of Breast Cancer-Derived Nanovesicles by Immunomagnetic Separation and Electrochemical Immunosensing-A Comparative Study. Sensors (Basel) 20:N/A (2020). PubMed: 32054015
  • Zhao L  et al. SGCE Promotes Breast Cancer Stem Cells by Stabilizing EGFR. Adv Sci (Weinh) 7:1903700 (2020). PubMed: 32714745
  • Sartages M  et al. High Levels of Receptor Tyrosine Kinases in CCM3-Deficient Cells Increase Their Susceptibility to Tyrosine Kinase Inhibition. Biomedicines 8:N/A (2020). PubMed: 33348877
  • Rodal-Cedeira S  et al. An Expanded Surface-Enhanced Raman Scattering Tags Library by Combinatorial Encapsulation of Reporter Molecules in Metal Nanoshells. ACS Nano 14:14655-14664 (2020). PubMed: 32869970
  • Kulukian A  et al. Preclinical Activity of HER2-Selective Tyrosine Kinase Inhibitor Tucatinib as a Single Agent or in Combination with Trastuzumab or Docetaxel in Solid Tumor Models. Mol Cancer Ther 19:976-987 (2020). PubMed: 32241871
View all Publications for this product

客户评价及客户问答

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1-8 of 8 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-EGFR antibody [EGFR1]

Average
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human tissue from different sites (TMA))
Antigen retrieval step
Other - Buffer/Enzyme Used: Citrate pH6, Tris-EDTA pH 9 and Chymotrypsin
Permeabilization
No
Specification
Human tissue from different sites (TMA)
Blocking step
(agent) for 10 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Malcolm Lim

Verified customer

提交于 Jul 14 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-EGFR antibody [EGFR1]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Breast cancer cell lines)
Permeabilization
Yes - 0.5% tritonx/PBS
Specification
Breast cancer cell lines
Blocking step
(agent) for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Malcolm Lim

Verified customer

提交于 Jul 05 2017

IHC - Wholemount abreview for Anti-EGFR antibody [EGFR1]

Good
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Human Tissue (Brain)
Specification
Brain
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 22 2013

Immunoprecipitation abreview for Anti-EGFR antibody [EGFR1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (sperm)
Total protein in input
1e+008 cells
Specification
sperm
Immuno-precipitation step
Protein A/G
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Yael Jaldety

Verified customer

提交于 Jun 24 2011

Question

Customer would like to know if these EGFR antibodies are specific for the truncated receptor.

Read More

Abcam community

Verified customer

Asked on May 24 2004

Answer

Regarding ab30, EGFR1 recognises the whole receptor protein rather than a truncated version.

Read More

Abcam Scientific Support

回复于 Jun 09 2004

Question

Customer would like to know if these EGFR antibodies are specific for the truncated receptor.

Read More

Abcam community

Verified customer

Asked on May 24 2004

Answer

In Western blotting ab231 detects a 170kDa band which corresponds to EGFR. The epitope recognized by this antibody is located on the extracellular domain of the EGFR. I belive that the truncated form of EGFR lacks the cytoplasmic and transmembrane domain. If it is indeed this simple, then I would expect the antibody to recognise the truncated form. However, to our knowledge, this antibody has not been tested for reactivity with this form of EGFR and therefore we would be unable to offer any guarantees.

Read More

Abcam Scientific Support

回复于 May 28 2004

Question

We are interested in purchasing the anti-EGFR antibody, Ab30, to use in immunohistochemistry experiments. My question relates to whether this antibody detects the mutant EGFR, EGFRvIII which has a deletion of a portion of the ECD. We are interested in antibodies which DO NOT pick up EGFRvIII.

Read More

Abcam community

Verified customer

Asked on Nov 20 2001

Answer

The antibody recognises the external EGF-binding domain of the EGFR transmembrane glycoprotein and the epitope was mapped to between aa 6-273 of human EGFR. We do not know which AA seq are deleted in EGFRvIII and therefore whether the epitope lies within the deleted region.

Read More

Danielle Miller

Abcam Scientific Support

回复于 Nov 21 2001

Question

you are the only purveyor of EGFR-1 (wild type vs VIII specific) that claims that it can be used on formalin-fixed, paraffin embedded tissue; all others producing it say it is not suitable for this application. Can you please tell me which antigen retrieval methods (protease, microwave, pressure cooker) you use with this Mab?

Read More

Abcam community

Verified customer

Asked on Feb 09 2001

Answer

In the following references the EGFR1 antibody has been used on paraffin-embedded sections: Ioachim E, et al. (1996) Anticancer Res 16(5B):3141-7. Nakopoulou L, et al. (1994) Nephrol Dial Transplant 9(7):764-9. Gorgoulis V, et al. (1992) Anticancer Res 12(4):1183-7.

Read More

Abcam Scientific Support

回复于 Feb 12 2001

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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