JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB8465

Anti-EGFR 抗体 [2E9]

Anti-EGFR antibody [2E9]

4

(2 Reviews)

|

(5 Publications)

Mouse Monoclonal EGFR antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human EGFR.

查看别名

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

2 Images
Flow Cytometry - Anti-EGFR antibody [2E9] (AB8465)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-EGFR antibody [2E9] (AB8465)

Overlay histogram showing A431 cells stained with ab8465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8465, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [2E9] (AB8465)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [2E9] (AB8465)

ICC/IF image of ab8465 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8465, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

2E9

亚型

IgG1

轻链类型

unknown

不含载体蛋白

No

反应种属

Human

应用

ICC/IF, Flow Cyt

applications

免疫原

Native Full Length Protein corresponding to Human EGFR.

P00533

特异性

This antibody reacts with a protein determinant of the extra cellular domain of the human EGFR. Shows binding competition with EGF and reacts in immunoprecipitation with the functional EFR protein-tyrosine kinase complex. The antibody has no agonistic properties, but competes efficiently with EGF for binding to the receptor

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.1 µg for 10^5 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "0.1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
style
left-column

产品详情

Sufficient for 100-200 tests
style
left-column

性能和储存信息

形式
Liquid
纯度
Tissue culture supernatant
纯化工艺
Affinity purification Protein G
存储溶液
pH: 7.3 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed : 10805725, PubMed : 27153536, PubMed : 2790960, PubMed : 35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed : 12297049, PubMed : 15611079, PubMed : 17909029, PubMed : 20837704, PubMed : 27153536, PubMed : 2790960, PubMed : 7679104, PubMed : 8144591, PubMed : 9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed : 27153536). May also activate the NF-kappa-B signaling cascade (PubMed : 11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed : 11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed : 11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed : 20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity).. Isoform 2 may act as an antagonist of EGF action.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.
See full target information EGFR
style
left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:2130 PubMed38503739

2024

Drug-resistant EGFR mutations promote lung cancer by stabilizing interfaces in ligand-free kinase-active EGFR oligomers.

Applications

Unspecified application

Species

Unspecified reactive species

R Sumanth Iyer,Sarah R Needham,Ioannis Galdadas,Benjamin M Davis,Selene K Roberts,Rico C H Man,Laura C Zanetti-Domingues,David T Clarke,Gilbert O Fruhwirth,Peter J Parker,Daniel J Rolfe,Francesco L Gervasio,Marisa L Martin-Fernandez

Journal of nanobiotechnology 15:73 PubMed29017600

2017

Ions doped melanin nanoparticle as a multiple imaging agent.

Applications

Unspecified application

Species

Unspecified reactive species

Shin-Woo Ha,Hee-Sang Cho,Young Il Yoon,Moon-Sun Jang,Kwan Soo Hong,Emmanuel Hui,Jung Hee Lee,Tae-Jong Yoon

Molecular therapy. Nucleic acids 6:89-105 PubMed28325303

2017

Inhibition of EGF Uptake by Nephrotoxic Antisense Drugs In Vitro and Implications for Preclinical Safety Profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Annie Moisan,Marcel Gubler,Jitao David Zhang,Yann Tessier,Kamille Dumong Erichsen,Sabine Sewing,Régine Gérard,Blandine Avignon,Sylwia Huber,Fethallah Benmansour,Xing Chen,Roberto Villaseñor,Annamaria Braendli-Baiocco,Matthias Festag,Andreas Maunz,Thomas Singer,Franz Schuler,Adrian B Roth

PloS one 8:e74200 PubMed24066121

2013

Hydrophobic fluorescent probes introduce artifacts into single molecule tracking experiments due to non-specific binding.

Applications

ICC/IF

Species

Unspecified reactive species

Laura C Zanetti-Domingues,Christopher J Tynan,Daniel J Rolfe,David T Clarke,Marisa Martin-Fernandez

The Journal of cell biology 109:2495-507 PubMed2553748

1989

Signal transduction by epidermal growth factor occurs through the subclass of high affinity receptors.

Applications

Unspecified application

Species

Unspecified reactive species

L H Defize,J Boonstra,J Meisenhelder,W Kruijer,L G Tertoolen,B C Tilly,T Hunter,P M van Bergen en Henegouwen,W H Moolenaar,S W de Laat
View all publications
style
left-column
style
left-column
style
right-column

替代产品

Primary Antibodies

AB32578

Anti-EGFR (phospho Y1173) antibody [E124]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124] (AB32578)

  • 0
  • 0
Primary Antibodies

AB40815

Anti-EGFR (phospho Y1068) antibody [EP774Y]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)

  • 4
  • 14
Primary Antibodies

AB76153

Anti-EGFR antibody [EPR39Y]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EPR39Y] (AB76153)

  • 0
  • 0
Primary Antibodies

AB32430

Anti-EGFR (phospho Y1068) antibody [Y38]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1068) antibody [Y38] (AB32430)

  • 0
  • 0
Primary Antibodies

AB32077

Anti-EGFR antibody [E235]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-EGFR antibody [E235] (AB32077)

  • 5
  • 4
Primary Antibodies

AB32562

Anti-EGFR antibody [E114]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-EGFR antibody [E114] (AB32562)

  • 0
  • 0
Primary Antibodies

AB227737

Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (AB227737)

  • 0
  • 0

搭配使用产品

Assay Kits

AB269558

Human soluble EGFR ELISA Kit

Recombinant|SimpleStep

ELISA - Human soluble EGFR ELISA Kit (AB269558)

  • 0
  • 0
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB134182

Anti-ErbB2 / HER2 antibody [EP1045Y]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (AB134182)

  • 4
  • 4

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com