重组Anti-EGF抗体[EPR19174] - Low endotoxin，Azide free (ab223549)
![Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)](https://www.abcam.cn/ps/products/223/ab223549/Images/ab223549-321422-anti-egf-antibody-epr19174-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19174] to EGF - Low endotoxin, Azide free
- Suitable for: IP, IHC-Fr, WB
- Reacts with: Mouse
概述
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产品名称
Anti-EGF抗体[EPR19174] - Low endotoxin，Azide free
参阅全部 EGF 一抗 -
描述
兔单克隆抗体[EPR19174] to EGF - Low endotoxin，Azide free -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-Fr, WBmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse EGF recombinant protein; Mouse salivary gland and kidney lysates; Mouse urine sample. IHC-Fr: Mouse kidney tissue. IP: Mouse urine sample
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常规说明
ab223549 is the carrier-free version of ab184266.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19174 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223549于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 160, 6 kDa (predicted molecular weight: 133 kDa).
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| 说明 |
|---|
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IP
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 160, 6 kDa (predicted molecular weight: 133 kDa). |
靶标
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功能
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). -
组织特异性
Expressed in kidney, salivary gland, cerebrum and prostate. -
疾病相关
Hypomagnesemia 4 -
序列相似性
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
翻译后修饰
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 13645 Mouse
- SwissProt: P01132 Mouse
- Unigene: 252481 Mouse
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别名
- Beta urogastrone antibody
- beta-urogastrone antibody
- EGF antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling EGF with ab184266 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green).
Weak staining on mouse liver is observed. The expression profile observed is consistent with what has been described in the literature (PMID:17671655).
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184266).
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![Immunoprecipitation - Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)](https://www.abcam.cn/ps/products/223/ab223549/Images/ab223549-321421-anti-egf-antibody-epr19174-immunoprecipitation.jpg)
Immunoprecipitation - Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)EGF was immunoprecipitated from 0.35 mg of Mouse urine sample with ab184266 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184266 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse urine sample, 10µg (Input).
Lane 2: ab184266 IP in Mouse urine sample.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184266 in Mouse urine sample.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184266).
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![Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)](https://www.abcam.cn/ps/products/223/ab223549/Images/ab223549-282801-anti-egf-antibody-epr19174-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)This IHC data was generated using the same anti-EGF antibody clone [EPR19174] in a different buffer formulation (cat# ab184266).
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling EGF with ab184266 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Cytoplasmic staining on mouse kidney is observed. The staining pattern is consistent with what has been described in the literature (PMID:17671655).
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)](https://www.abcam.cn/ps/products/223/ab223549/Images/ab223549-4-benefits-of-recombinant-antibodies.png)
Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)
实验方案
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
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