Anti-Eg5抗体[mAbcam 51976] (ab51976)
![Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [mAbcam 51976] (ab51976)](https://www.abcam.cn/ps/products/51/ab51976/Images/ab51976-19-anti-eg5-mabcam-51976-antibody-immunocytochemistry-hela-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [mAbcam 51976] (ab51976)")
Key features and details
- Mouse monoclonal [mAbcam 51976] to Eg5
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
-
产品名称
Anti-Eg5抗体[mAbcam 51976]
参阅全部 Eg5 一抗 -
描述
小鼠单克隆抗体[mAbcam 51976] to Eg5 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Eg5 aa 950-1050 conjugated to keyhole limpet haemocyanin.
(Peptide available asab38094) -
阳性对照
- This antibody gave a positive signal in the following whole cell lysates: HEK293, Jurkat and A431.
-
常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
mAbcam 51976 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51976于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 1 µg/ml.
|
| WB | (1) |
Use a concentration of 5 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
|
| IP |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa). |
|
IP
Use at an assay dependent concentration. |
靶标
-
功能
Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays. -
疾病相关
Defects in KIF11 are the cause of microcephaly with or without chorioretinopathy, lymphedema, or mental retardation (MCLMR) [MIM:152950]. An autosomal dominant disorder that involves an overlapping but variable spectrum of central nervous system and ocular developmental anomalies. Microcephaly ranges from mild to severe and is often associated with mild to moderate developmental delay and a characteristic facial phenotype with upslanting palpebral fissures, broad nose with rounded tip, long philtrum with thin upper lip, prominent chin, and prominent ears. Chorioretinopathy is the most common eye abnormality, but retinal folds, microphthalmia, and myopic and hypermetropic astigmatism have also been reported, and some individuals have no overt ocular phenotype. Congenital lymphedema, when present, is typically confined to the dorsa of the feet, and lymphoscintigraphy reveals the absence of radioactive isotope uptake from the webspaces between the toes. -
序列相似性
Belongs to the kinesin-like protein family. BimC subfamily.
Contains 1 kinesin-motor domain. -
翻译后修饰
Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. - Information by UniProt
-
数据库链接
- Entrez Gene: 3832 Human
- Omim: 148760 Human
- SwissProt: P52732 Human
- Unigene: 8878 Human
-
别名
- EG5 antibody
- HKSP antibody
- KIF11 antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [mAbcam 51976] (ab51976)ab51976 staining Eg5 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab51976 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
-
![Western blot - Anti-Eg5 antibody [mAbcam 51976] (ab51976)](https://www.abcam.cn/ps/products/51/ab51976/Images/ab51976-64028-anti-eg5-antibody-mabcam-51976-western-blot.jpg)
Western blot - Anti-Eg5 antibody [mAbcam 51976] (ab51976)All lanes : Anti-Eg5 antibody [mAbcam 51976] (ab51976) at 5 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Mouse IgG - H&L (HRP) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 120 kDa -
![Immunoprecipitation - Anti-Eg5 antibody [mAbcam 51976] (ab51976)](https://www.abcam.cn/ps/products/51/ab51976/Images/ab51976-64025-anti-eg5-antibody-mabcam-51976-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Eg5 antibody [mAbcam 51976] (ab51976)Eg5 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Mouse monoclonal to Eg5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab51976.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 120kDa: Eg5
实验方案
数据表及文件
-
Datasheet download
文献 (8)
ab51976 被引用在 8 文献中.
- Zheng J et al. NAT10 regulates mitotic cell fate by acetylating Eg5 to control bipolar spindle assembly and chromosome segregation. Cell Death Differ 29:846-860 (2022). PubMed: 35210604
- Murase Y et al. Inhibitor library screening identifies ispinesib as a new potential chemotherapeutic agent for pancreatic cancers. Cancer Sci 112:4641-4654 (2021). PubMed: 34510663
- Wu W et al. S-trityl-L-cysteine, a novel Eg5 inhibitor, is a potent chemotherapeutic strategy in neuroblastoma. Oncol Lett 16:1023-1030 (2018). PubMed: 29963178
- Swarnkar S et al. Kinesin Family of Proteins Kif11 and Kif21B Act as Inhibitory Constraints of Excitatory Synaptic Transmission Through Distinct Mechanisms. Sci Rep 8:17419 (2018). PubMed: 30479371
- Chien JY et al. aTAT1 downregulation induces mitotic catastrophe in HeLa and A549 cells. Cell Death Discov 2:16006 (2016). PubMed: 27551500
- Akera T et al. Mad1 promotes chromosome congression by anchoring a kinesin motor to the kinetochore. Nat Cell Biol 17:1124-33 (2015). PubMed: 26258632
- Drosopoulos K et al. APC/C is an essential regulator of centrosome clustering. Nat Commun 5:3686 (2014). PubMed: 24751481
- Dai J et al. Studies of haspin-depleted cells reveal that spindle-pole integrity in mitosis requires chromosome cohesion. J Cell Sci 122:4168-76 (2009). ICC/IF ; Human . PubMed: 19910498
