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重组RabMAb

重组Anti-Eg5抗体[EPR23277-7] - BSA and Azide free (ab272226)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
  • Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
  • Flow Cytometry (Intracellular) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
  • Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
  • Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23277-7] to Eg5 - BSA and Azide free
  • Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Eg5抗体[EPR23277-7] - BSA and Azide free
    参阅全部 Eg5 一抗

  • 描述

    兔单克隆抗体[EPR23277-7] to Eg5 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details
    不适用于: IHC-Fr or IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Human testis, human tonsil, human lung, Jurkat, NCI-H1975, MCF7, 4T1, HeLa, HEK-293, AR42J, mouse testis, rat testis and PC-12, NIH/3T3 lysates. IHC-P: Human tonsil, human breast cancer and mouse testis, Rat testis tissues. ICC/IF: and HeLa, NIH/3T3 cells. Flow Cyt (intra): HeLa cell.

  • 常规说明

    ab272226 is the carrier-free version of ab272220.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272226于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 119 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
WB
Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 119 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
应用说明
Is unsuitable for IHC-Fr or IP.

靶标

  • 功能

    Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.

  • 疾病相关

    Defects in KIF11 are the cause of microcephaly with or without chorioretinopathy, lymphedema, or mental retardation (MCLMR) [MIM:152950]. An autosomal dominant disorder that involves an overlapping but variable spectrum of central nervous system and ocular developmental anomalies. Microcephaly ranges from mild to severe and is often associated with mild to moderate developmental delay and a characteristic facial phenotype with upslanting palpebral fissures, broad nose with rounded tip, long philtrum with thin upper lip, prominent chin, and prominent ears. Chorioretinopathy is the most common eye abnormality, but retinal folds, microphthalmia, and myopic and hypermetropic astigmatism have also been reported, and some individuals have no overt ocular phenotype. Congenital lymphedema, when present, is typically confined to the dorsa of the feet, and lymphoscintigraphy reveals the absence of radioactive isotope uptake from the webspaces between the toes.

  • 序列相似性

    Belongs to the kinesin-like protein family. BimC subfamily.
    Contains 1 kinesin-motor domain.

  • 翻译后修饰

    Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
    A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.

  • 细胞定位

    Cytoplasm. Cytoplasm > cytoskeleton > spindle pole.
  • Information by UniProt

  • 数据库链接

    • 别名

      • EG5 antibody
      • HKSP antibody
      • KIF11 antibody
      • KIF11_HUMAN antibody
      • Kinesin family member 11 antibody
      • Kinesin like protein 1 antibody
      • Kinesin-like protein 1 antibody
      • Kinesin-like protein KIF11 antibody
      • Kinesin-like spindle protein HKSP antibody
      • Kinesin-related motor protein Eg5 antibody
      • KNSL1 antibody
      • MCLMR antibody
      • Thyroid receptor-interacting protein 5 antibody
      • TR-interacting protein 5 antibody
      • TRIP-5 antibody
      • TRIP5 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Eg5 with ab272220 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in germinal center of human tonsil is observed (PMID:25277178). The section was incubated with ab272220 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272220).

    • Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
      Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling Eg5 with ab272220 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing spindle fibre and cytoplasmic staining in HeLa cell line. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272220).

    • Flow Cytometry (Intracellular) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
      Flow Cytometry (Intracellular) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)

      Intracellular flow cytometric analysis of 80% methanol fixed 0.1% Tween-20 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Eg5 with ab272220 at 1/50 dilution (0.1µg)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were pre-treated with 20µg/ml RNaseA for 30min to minimize the binding between Propidium Iodide (PI) and RNA. Then intracellularly stained with ab272220 and PI. Propidium Iodide Flow Cytometry Kit (ab139418) was used for RNaseA treatment and PI staining. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272220). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)

      Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Eg5 with ab272220 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in spermatocytes and spermatogonnia of mouse testis is observed. The section was incubated with ab272220 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272220).

    • Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
      Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labeling Eg5 with ab272220 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing spindle fibre and cytoplasmic staining in NIH/3T3 cell line. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272220).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)

      Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Eg5 with ab272220 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in spermatocytes and spermatogonnia of rat testis is observed. The section was incubated with ab272220 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272220).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)

      Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Eg5 with ab272220 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Scattered cytoplasmic staining in human breast cancer cells is observed (PMID:29181100). The section was incubated with ab272220 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272220).

    • Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
      Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab272226？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab272226 尚未被引用在任何文献中。

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