Anti-EFTUD2抗体(ab72456)
Key features and details
- Rabbit polyclonal to EFTUD2
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-EFTUD2抗体
参阅全部 EFTUD2 一抗 -
描述
兔多克隆抗体to EFTUD2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Caenorhabditis elegans, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide (Human) corresponding to a region between residues 1 and 50 of EFTUD2. (NP_004238.2)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72456于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 109 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 109 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP. -
疾病相关
Defects in EFTUD2 are the cause of mandibulofacial dysostosis with microcephaly (MFDM) [MIM:610536]. A rare syndrome characterized by progressive microcephaly, midface and malar hypoplasia, micrognathia, microtia, dysplastic ears, preauricular skin tags, significant developmental delay, and speech delay. Many patients have major sequelae, including choanal atresia that results in respiratory difficulties, conductive hearing loss, and cleft palate. -
序列相似性
Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 428281 Chicken
- Entrez Gene: 509425 Cow
- Entrez Gene: 9343 Human
- Entrez Gene: 20624 Mouse
- Entrez Gene: 287739 Rat
- Entrez Gene: 379223 Xenopus laevis
- Entrez Gene: 594937 Xenopus tropicalis
- Entrez Gene: 393480 Zebrafish
see all -
别名
- 116 kDa antibody
- 116 kDa U5 small nuclear ribonucleoprotein component antibody
- EFTUD2 antibody
see all
图片
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Western blot - Anti-EFTUD2 antibody (ab72456)All lanes : Anti-EFTUD2 antibody (ab72456) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 109 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFTUD2 antibody (ab72456)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling EFTUD2 with ab72456 at 1/200 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-EFTUD2 antibody (ab72456)HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), with ab72456 used for IP at 3 µg/mg lysate, and at 0.1 µg/ml for subsequent WB.
Detection: Chemiluminescence with an exposure time of 10 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFTUD2 antibody (ab72456)IHC image of ab72456 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72456, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab72456 被引用在 5 文献中.
- Yang GC et al. Spliceosomal GTPase Eftud2 regulates microglial activation and polarization. Neural Regen Res 18:856-862 (2023). PubMed: 36204854
- Serna M et al. CryoEM of RUVBL1-RUVBL2-ZNHIT2, a complex that interacts with pre-mRNA-processing-splicing factor 8. Nucleic Acids Res 50:1128-1146 (2022). PubMed: 34951455
- Beyer S et al. High RIG-I and EFTUD2 expression predicts poor survival in endometrial cancer. J Cancer Res Clin Oncol N/A:N/A (2022). PubMed: 36068443
- Thillainadesan G et al. Conserved protein Pir2ARS2 mediates gene repression through cryptic introns in lncRNAs. Nat Commun 11:2412 (2020). PubMed: 32415063
- Cloutier P et al. R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein. Nat Commun 8:15615 (2017). WB . PubMed: 28561026