AB151976

Anti-EFEMP1/Fibulin-3 抗体

Name: EFEMP1/Fibulin-3抗体 (ab151976)| Abcam中文官网
Brand: Abcam Limited
SKU: AB151976
Price: 4606 CNY
Availability: InStock

Anti-EFEMP1/Fibulin-3 antibody

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal EFEMP1/Fibulin-3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Recombinant fragment samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EFEMP1 aa 150 to C-terminus.

查看别名

FBLN3, FBNL, EFEMP1, EGF-containing fibulin-like extracellular matrix protein 1, Extracellular protein S1-5, Fibrillin-like protein, Fibulin-3, FIBL-3

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Immunohistochemical analysis of paraffin embedded NCIN87 xenograft tissue labeling EFEMP1/Fibulin-3 with ab151976 at a 1/500 dilution.

Unknown

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

10% SDS PAGE

All lanes:

Western blot - Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/1000 dilution

Lane 1:

HEK-293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

H1299 whole cell lysate at 30 µg

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/1000 dilution

Lane 1:

HUVEC whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/1000 dilution

Lane 1:

Mouse lung tissue extracts at 50 µg

Lane 2:

Mouse testis tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 54 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human, Mouse

应用

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human EFEMP1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q12805

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Recombinant fragment": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EFEMP1 also known as Fibulin-3 is a member of the fibulin family involved in extracellular matrix (ECM) organization. The protein's mass is approximately 55 kDa. It is expressed in various tissues but researchers find it most common in the eye lung and arterial walls. As a secreted glycoprotein Fibulin-3 plays a vital role in maintaining structural integrity and elasticity in connective tissues.

Biological function summary

EFEMP1/Fibulin-3 participates in ECM modulation and cell signaling. It interacts with other ECM components such as fibronectin and collagen contributing to structural assembly and stability. Although not prominently part of multi-protein complexes Fibulin-3 influences cellular adhesion and migration impacting tissue development and repair mechanisms. Researchers observe its expression during embryogenesis suggesting a role in developmental processes.

Pathways

EFEMP1/Fibulin-3 integrates into matrix-related pathways impacting tissue architecture. It plays a role in the EGF (Epidermal Growth Factor) signaling pathway which is important for various cell survival and proliferation processes. Fibulin-3 exhibits functional relationships with proteins like fibronectin and integrins within these pathways highlighting its importance in mediating cell-matrix interactions and influencing signal transduction cascades.

EFEMP1/Fibulin-3 has notable connections with certain pathologies. Malfunctions in its expression or structure can relate to age-related macular degeneration and various forms of cancer including mesothelioma. In these diseases Fibulin-3 interacts with proteins such as matrix metalloproteinases which degrade extracellular matrix proteins thereby influencing tumor progression and ECM remodeling in affected tissues.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.

See full target information EFEMP1

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 14:e1007532 PubMed30102696

2018

A genome-wide association study identifies a susceptibility locus for biliary atresia on 2p16.1 within the gene EFEMP1.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Chen,Melissa A Gilbert,Christopher M Grochowski,Deborah McEldrew,Jessica Llewellyn,Orith Waisbourd-Zinman,Hakon Hakonarson,Joan E Bailey-Wilson,Pierre Russo,Rebecca G Wells,Kathleen M Loomes,Nancy B Spinner,Marcella Devoto

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EFEMP1/Fibulin-3 抗体

Anti-EFEMP1/Fibulin-3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)