Anti-EEF2K (phospho S366)抗体(ab51227)
Key features and details
- Rabbit polyclonal to EEF2K (phospho S366)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-EEF2K (phospho S366)抗体
参阅全部 EEF2K 一抗 -
描述
兔多克隆抗体to EEF2K (phospho S366) -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human EEF2K aa 300-400 (phospho S366).
Database link: O00418 -
阳性对照
- Hela cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51227于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
1/50 - 1/100.
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| WB |
1/300 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 95 kDa).
|
| 说明 |
|---|
|
IHC-P
1/50 - 1/100. |
|
WB
1/300 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 95 kDa). |
靶标
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功能
Phosphorylates eukaryotic elongation factor-2. Binds calmodulin. -
序列相似性
Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 29904 Human
- Entrez Gene: 13631 Mouse
- Entrez Gene: 25435 Rat
- Omim: 606968 Human
- SwissProt: O00418 Human
- SwissProt: O08796 Mouse
- SwissProt: P70531 Rat
- Unigene: 498892 Human
see all -
别名
- C86191 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
see all
图片
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Western blot - Anti-EEF2K (phospho S366) antibody (ab51227)All lanes : Anti-EEF2K (phospho S366) antibody (ab51227) at 1/300 dilution
Lane 1 : Extracts of Hela cells treated with 10% serum for 15mins.
Lane 2 : Extracts of Hela cells treated with 10% serum for 15mins and phosphopeptide.
Predicted band size: 95 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S366) antibody (ab51227)Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab51227 (right picture is blocked with the phospho peptide)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab51227 尚未被引用在任何文献中。