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AB283706

重组Anti-EEF2K抗体[EPR24714-88] - BSA and Azide free

Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • KO Validated
  • 了解详情

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Rabbit Recombinant Monoclonal EEF2K antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), WB, IHC-P and reacts with Human samples.

查看别名

Eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, EEF2K

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)

This data was developed using ab270948, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling EEF2K with ab270948 at 1/500 (1.178 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human pancreatic acinar cells. The section was incubated with ab270948 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)

This data was developed using ab270948, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling EEF2K with ab270948 at 1/50 dilution (1ug)/(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)

This data was developed using ab270948, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling EEF2K with ab270948 at 1/500 (1.178 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining on human liver. The section was incubated with ab270948 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)

This data was developed using ab270948, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) cells labelling EEF2K with ab270948 at 1/50 dilution (1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)

This data was developed using ab270948, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling EEF2K with ab270948 at 1/500 (1.178 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human breast carcinoma. The section was incubated with ab270948 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)

This data was developed using ab270948, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labelling EEF2K with ab270948 at 1/500 (1.178 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human endometrial carcinoma. The section was incubated with ab270948 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunoprecipitation - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)
  • IP

Supplier Data

Immunoprecipitation - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)

This data was developed using ab270948, the same antibody clone in a different buffer formulation.

EEF2K was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab270948 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270948 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug

Lane 2 : ab270948 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab270948 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 seconds

All lanes:

Immunoprecipitation - Anti-EEF2K antibody [EPR24714-88] (<a href='/products/primary-antibodies/eef2k-antibody-epr24714-88-ab270948'>ab270948</a>)

Predicted band size: 82 kDa

false

Western blot - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)
  • WB

Lab

Western blot - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)

This data was developed using ab270948, the same antibody clone in a different buffer formulation.

Western blot : Rabbit Monoclonal[EPR24714-88] to EEF2K ab270948 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 82 kDa in Wild-type A549 cell lysates with no signal observed at this size in EEF2K knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

Lanes 1 - 4:

Western blot - Anti-EEF2K antibody [EPR24714-88] (<a href='/products/primary-antibodies/eef2k-antibody-epr24714-88-ab270948'>ab270948</a>) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (ab283706) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

EEF2K knockout A549 at 20 µg

Lane 3:

Raji at 20 µg

Lane 4:

MOLT-4 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 82 kDa

Observed band size: 82 kDa

false

Western blot - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)
  • WB

Lab

Western blot - Anti-EEF2K antibody [EPR24714-88] - BSA and Azide free (AB283706)

This data was developed using ab270948, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Lysates were made freshly and used in WB test immediately to minimize protein degradation.

Exposure time : 10 seconds

All lanes:

Western blot - Anti-EEF2K antibody [EPR24714-88] (<a href='/products/primary-antibodies/eef2k-antibody-epr24714-88-ab270948'>ab270948</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 82 kDa

Observed band size: 95 kDa

false

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR24714-88

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

Flow Cyt (Intra), WB, IP, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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产品详情

ab283706 is the carrier-free version of ab270948.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EEF2K also known as eukaryotic elongation factor 2 kinase or CaMKIII is a significant protein kinase with an approximate molecular mass of 85 kDa. EEF2K plays a role in controlling protein synthesis by phosphorylating eukaryotic elongation factor 2 (eEF2) leading to a decrease in its activity. This protein can be found in various tissues throughout the body but shows substantial expression in the brain. It acts as a regulatory switch in translation process under specific cellular conditions.
Biological function summary

EEF2K regulates protein translation and affects cellular energy expenditure during conditions such as starvation or stress. Through its activity EEF2K contributes to the cellular response by phosphorylating eEF2 resulting in modulation of ribosomal function. Although EEF2K does not necessarily form stable complexes with other proteins its function influences several other cellular activities that are in connection with protein synthesis and degradation pathways.

Pathways

EEF2K integrates into nutrient-signaling pathways like the mTOR signaling and the AMPK pathway. These pathways control energy homeostasis and response to cellular stress. EEF2K acts alongside AMPK when nutrient supply is low adjusting protein synthesis rates to meet cellular energy demands. Additionally EEF2K's function inversely relates to mTOR activity which typically promotes protein synthesis and cell growth when conditions are favorable.

EEF2K associates with cancer and neurodegenerative diseases. In cancer EEF2K modulates cell survival under nutrient-poor environments often contributing to chemotherapy resistance. Links between EEF2K and neurodegenerative diseases come from its regulation of protein synthesis which affects neuronal health. Proteins like AMPK connect to EEF2K highlighting their collective contribution to the metabolic rearrangements seen in these disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
See full target information Eukaryotic elongation factor 2 kinase
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搭配使用产品

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