Anti-EEF2/Elongation factor 2 (phospho T56)抗体(ab53114)
Key features and details
- Rabbit polyclonal to EEF2/Elongation factor 2 (phospho T56)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-EEF2/Elongation factor 2 (phospho T56)抗体
参阅全部 EEF2/Elongation factor 2 一抗 -
描述
兔多克隆抗体to EEF2/Elongation factor 2 (phospho T56) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human EEF2/Elongation factor 2 aa 1-100 (phospho T56).
Database link: P13639 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab53114 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53114于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/300 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
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| IHC-P |
Use at an assay dependent concentration.
|
| 说明 |
|---|
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WB
1/300 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. -
序列相似性
Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily. -
翻译后修饰
Phosphorylation by EF-2 kinase completely inactivates EF-2.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
ISGylated. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1938 Human
- Entrez Gene: 13629 Mouse
- Omim: 130610 Human
- SwissProt: P13639 Human
- SwissProt: P58252 Mouse
- Unigene: 515070 Human
- Unigene: 326799 Mouse
- Unigene: 379638 Mouse
see all -
别名
- EEF 2 antibody
- Eef2 antibody
- EF-2 antibody
see all
图片
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Western blot - Anti-EEF2/Elongation factor 2 (phospho T56) antibody (ab53114)All lanes : Anti-EEF2/Elongation factor 2 (phospho T56) antibody (ab53114) at 1/300 dilution
Lane 1 : Extract from NIH 3T3 cells
treated with serum (10%, 30mins) with Immunising peptide
Lane 2 : Extract from NIH 3T3 cells
treated with serum (10%, 30mins)
Predicted band size: 95 kDa
Observed band size: 95 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 (phospho T56) antibody (ab53114)ab53114 at 1/50 dilution staining EEF2/Elongation factor 2 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab53114 被引用在 5 文献中.
- Taha E et al. eEF2/eEF2K Pathway in the Mature Dentate Gyrus Determines Neurogenesis Level and Cognition. Curr Biol 30:3507-3521.e7 (2020). PubMed: 32707059
- Ma YF et al. Phosphorylation of AKT serine/threonine kinase and abundance of milk protein synthesis gene networks in mammary tissue in response to supply of methionine in periparturient Holstein cows. J Dairy Sci 102:4264-4274 (2019). PubMed: 30879806
- Delaidelli A et al. MYCN amplified neuroblastoma requires the mRNA translation regulator eEF2 kinase to adapt to nutrient deprivation. Cell Death Differ 24:1564-1576 (2017). PubMed: 28574509
- Grandjean M et al. Hypoxia Integration in the Serological Proteome Analysis Unmasks Tumor Antigens and Fosters the Identification of Anti-Phospho-eEF2 Antibodies as Potential Cancer Biomarkers. PLoS One 8:e76508 (2013). WB, ICC/IF ; Mouse, Human . PubMed: 24130777
- Collodoro M et al. Identification and quantification of concentration-dependent biomarkers in MCF-7/BOS cells exposed to 17ß-estradiol by 2-D DIGE and label-free proteomics. J Proteomics 75:4555-69 (2012). WB ; Human . PubMed: 22580036