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Anti-EEF2/Elongation factor 2抗体[4B3-G7-H5] (ab131202)

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Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
  • Immunocytochemistry/ Immunofluorescence - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
  • Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
  • Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)

Key features and details

  • Mouse monoclonal [4B3-G7-H5] to EEF2/Elongation factor 2
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG2b

概述

  • 产品名称

    Anti-EEF2/Elongation factor 2抗体[4B3-G7-H5]
    参阅全部 EEF2/Elongation factor 2 一抗

  • 描述

    小鼠单克隆抗体[4B3-G7-H5] to EEF2/Elongation factor 2

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC/IF, WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Human, African green monkey

  • 免疫原

    Recombinant fragment corresponding to Human EEF2/Elongation factor 2.
    Database link: P13639

  • 阳性对照

    • HL-60 cell lysate

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 17.08.2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.40
    Preservative: 0.03% Proclin 300
    Constituent: 50% Glycerol
  • Concentration information loading...

  • 纯度

    Concentrated Culture Supernatant

  • 克隆

    单克隆

  • 克隆编号

    4B3-G7-H5

  • 同种型

    IgG2b

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab131202于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.
WB (1)
1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
说明
ICC/IF
Use at an assay dependent concentration.
WB
1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).

靶标

  • 功能

    Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

  • 序列相似性

    Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

  • 翻译后修饰

    Phosphorylation by EF-2 kinase completely inactivates EF-2.
    Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
    ISGylated.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • EEF 2 antibody
    • Eef2 antibody
    • EF-2 antibody
    • EF2 antibody
    • EF2_HUMAN antibody
    • Elongation factor 2 antibody
    • Eukaryotic translation elongation factor 2 antibody
    • Polypeptidyl tRNA translocase antibody
    • SCA26 antibody
    see all

图片

  • Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
    Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
    All lanes : Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202) at 1/5000 dilution

    Lane 1 : Jurkat Cell lysates
    Lane 2 : C6 Cell lysates
    Lane 3 : 3T3 Cell lysates
    Lane 4 : COS-7 (African green monkey kidney fibroblast-like cell line) Cell lysates
    Lane 5 : HeLa Cell lysates

    Predicted band size: 95 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
    Immunocytochemistry/ Immunofluorescence - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)

    Immunocytochemical staining of HeLa cells using anti-eEF2/Elongation factor 2 antibody (ab131202) at a dilution of 1:200. Cells were fixed with ice cold methanol. This image was produced with antibody produced in ascites. 

  • Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
    Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)

    Western blot detection of EEF2/Elongation factor 2 in HL-60,Jurkat,SHSY-5Y,U20S and Hela cell lysates using EEF2/Elongation factor 2 mouse mAb (1:5000).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s. This image was produced with antibody produced in ascites. 

  • Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
    Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
    Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202) at 1/1000 dilution + HL-60 cell lysate

    Predicted band size: 95 kDa
    Observed band size: 95 kDa



    This image was produced with antibody produced in ascites. 

文献 (0)

发表研究结果有使用 ab131202？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab131202 尚未被引用在任何文献中。

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Western blot abreview for Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
HEK293 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Melanie Thelen

Verified customer

提交于 Apr 06 2016

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