Anti-eEF1B2抗体(ab37725)
Key features and details
- Rabbit polyclonal to eEF1B2
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-eEF1B2抗体
参阅全部 eEF1B2 一抗 -
描述
兔多克隆抗体to eEF1B2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rabbit, Cow, Pig
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human eEF1B2.
参阅Abcam的专有抗源政策 (Peptide available as ab37955.) -
阳性对照
- HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate, HEK 293 (Human embryonic kidney cell line) Whole Cell Lysate, HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate, MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate and U2OS (Human osteosarcoma cell line) Whole Cell Lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab37725于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/250. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
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| IHC-P |
Use a concentration of 1 µg/ml.
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
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WB
1/250. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa). |
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IHC-P
Use a concentration of 1 µg/ml. |
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ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. -
序列相似性
Belongs to the EF-1-beta/EF-1-delta family.
Contains 1 GST C-terminal domain. -
翻译后修饰
Phosphorylation affects the GDP/GTP exchange rate. - Information by UniProt
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数据库链接
- Entrez Gene: 1933 Human
- Entrez Gene: 55949 Mouse
- Omim: 600655 Human
- SwissProt: P24534 Human
- SwissProt: O70251 Mouse
- Unigene: 421608 Human
- Unigene: 2718 Mouse
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别名
- EEF1B antibody
- EEF1B1 antibody
- EEF1B2 antibody
see all
图片
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Western blot - Anti-eEF1B2 antibody (ab37725)All lanes : Anti-eEF1B2 antibody (ab37725) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : EEF1B2 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab37725 observed at 30 kDa. Red - loading control ab7291 observed at 50 kDa.
ab37725 Anti-eEF1B2 antibody was shown to specifically react with eEF1B2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266769 (knockout cell lysate ab257934) was used. Wild-type and eEF1B2 knockout samples were subjected to SDS-PAGE. ab37725 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence - Anti-eEF1B2 antibody (ab37725)ICC/IF image of ab37725 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37725, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, HepG2 and Hek293 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1B2 antibody (ab37725)IHC image of ab37725 staining eEF1B2 in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37725, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-eEF1B2 antibody (ab37725)All lanes : Anti-eEF1B2 antibody (ab37725) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lane 4 :HEK-293 whole cell lysate (ab7902)
Lane 5 : Hep G2 whole cell lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab37725 被引用在 3 文献中.
- Jia L et al. Translation elongation factor eEF1Ba is identified as a novel prognostic marker of gastric cancer. Int J Biol Macromol 126:345-351 (2018). PubMed: 30572058
- Sivan G et al. Mitotic modulation of translation elongation factor 1 leads to hindered tRNA delivery to ribosomes. J Biol Chem 286:27927-35 (2011). WB ; Human . PubMed: 21665947
- Byun HO et al. Cathepsin D and eukaryotic translation elongation factor 1 as promising markers of cellular senescence. Cancer Res 69:4638-47 (2009). WB ; Human . PubMed: 19487283