Anti-EED抗体(ab4469)
Key features and details
- Rabbit polyclonal to EED
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-EED抗体
参阅全部 EED 一抗 -
描述
兔多克隆抗体to EED -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human, Recombinant fragment
预测可用于: Mouse, Chicken, Cow, Xenopus laevis
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human Recombinant EED Protein, HAP1, HeLa and HepG2 cell lysates ICC/IF: U-2 OS cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
1/200. Predicted molecular weight: 49 kDa.
|
| ICC/IF |
1/100.
|
| 说明 |
|---|
|
WB
1/200. Predicted molecular weight: 49 kDa. |
|
ICC/IF
1/100. |
靶标
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功能
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. -
组织特异性
Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer. -
序列相似性
Belongs to the WD repeat ESC family.
Contains 7 WD repeats. -
发展阶段
Expression peaks at the G1/S phase boundary. -
细胞定位
Nucleus. Chromosome. Transiently colocalizes with XIST at inactive X chromosomes. - Information by UniProt
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数据库链接
- Entrez Gene: 426381 Chicken
- Entrez Gene: 404183 Cow
- Entrez Gene: 8726 Human
- Entrez Gene: 13626 Mouse
- Entrez Gene: 398420 Xenopus laevis
- Omim: 605984 Human
- SwissProt: Q5ZKH3 Chicken
- SwissProt: Q3SZ25 Cow
see all -
别名
- eed antibody
- EED protein antibody
- EED, mouse, homolog of antibody
see all
图片
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Western blot - Anti-EED antibody (ab4469)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: EED knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab4469 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab4469 was shown to recognize EED in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when EED knockout samples were examined. Wild-type and EED knockout samples were subjected to SDS-PAGE. ab4469 and ab8245 (loading control to GAPDH) were diluted 1/250 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
Immunocytochemistry/ Immunofluorescence - Anti-EED antibody (ab4469)Rabbit polyclonal to EED (ab4469) on U-2 OS (Human bone osteosarcoma epithelial cell line) cells. Antibody used at 1/100.
U-2 OS cells were fixed with PFA (3.7%), permeabilised with PBS 0.1% Triton. Counterstained with Hoechst. Cells were examined with Axioplan 2 microscope (Zeiss).
Secondary antibody: donkey anti-rabbit (Cy3). -
Western blot - Anti-EED antibody (ab4469)Anti-EED antibody (ab4469) at 1/250 dilution + EED Recombinant Protein (Human) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
ab4469 recognizes the full-length recombinant EED protein which has an expected molecular weight of 80 kDa (tagged).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (31)
ab4469 被引用在 31 文献中.
- Deshayes F et al. Link between the EZH2 noncanonical pathway and microtubule organization center polarization during early T lymphopoiesis. Sci Rep 12:3655 (2022). PubMed: 35256668
- Gonzalez ME et al. EZH2 T367 phosphorylation activates p38 signaling through lysine methylation to promote breast cancer progression. iScience 25:104827 (2022). PubMed: 35992062
- Boulay G et al. The chromatin landscape of primary synovial sarcoma organoids is linked to specific epigenetic mechanisms and dependencies. Life Sci Alliance 4:N/A (2021). PubMed: 33361335
- Ngan CY et al. Chromatin interaction analyses elucidate the roles of PRC2-bound silencers in mouse development. Nat Genet 52:264-272 (2020). PubMed: 32094912
- Ma P et al. RNF220 is required for cerebellum development and regulates medulloblastoma progression through epigenetic modulation of Shh signaling. Development 147:N/A (2020). PubMed: 32376680