Anti-EEA1抗体[1G11] - Early Endosome Marker (ab70521)
![Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)](https://www.abcam.cn/ps/products/70/ab70521/Images/ab70521-18-anti-eea1-1g11-antibody-immunocytochemistry-hepg2-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)")
Key features and details
- Mouse monoclonal [1G11] to EEA1 - Early Endosome Marker
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-EEA1抗体[1G11] - Early Endosome Marker
参阅全部 EEA1 一抗 -
描述
小鼠单克隆抗体[1G11] to EEA1 - Early Endosome Marker -
宿主
Mouse -
经测试应用
适用于: ICC/IFmore details
不适用于: WB -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit
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免疫原
Recombinant fragment corresponding to Human EEA1 aa 50 to the C-terminus.
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阳性对照
- This anitbody gave a positive immunofluorescent signal in the following cell lines: HeLa HepG2
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
1G11 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70521于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (6) |
Use a concentration of 1 - 5 µg/ml.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
应用说明
Is unsuitable for WB.
靶标
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功能
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. -
序列相似性
Contains 1 C2H2-type zinc finger.
Contains 1 FYVE-type zinc finger. -
结构域
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions. -
细胞定位
Cytoplasm. Early endosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8411 Human
- Entrez Gene: 216238 Mouse
- Entrez Gene: 314764 Rat
- Omim: 605070 Human
- SwissProt: Q15075 Human
- SwissProt: Q8BL66 Mouse
- Unigene: 567367 Human
- Unigene: 210035 Mouse
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别名
- Early endosome antigen 1 antibody
- Early endosome antigen 1, 162kD antibody
- Early endosome associated protein antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)ab70521 staining EEA1 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab70521 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 100% methanol (5 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
数据表及文件
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SDS download
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Datasheet download
文献 (77)
ab70521 被引用在 77 文献中.
- Lam KK et al. KRAS-specific antibody binds to KRAS protein inside colorectal adenocarcinoma cells and inhibits its localization to the plasma membrane. Front Oncol 13:1036871 (2023). PubMed: 37051535
- Nyati S et al. Budding uninhibited by benzimidazoles-1 (BUB1) regulates EGFR signaling by reducing EGFR internalization. Aging (Albany NY) 15:6011-6030 (2023). PubMed: 37399454
- Zhan Z et al. Exclusion of NUMB Exon12 Controls Cancer Cell Migration through Regulation of Notch1-SMAD3 Crosstalk. Int J Mol Sci 23:N/A (2022). PubMed: 35457181
- Jiang C et al. Spatiotemporal three-dimensional transport dynamics of endocytic cargos and their physical regulations in cells. iScience 25:104210 (2022). PubMed: 35479412
- Zeziulia M et al. Proton-gated anion transport governs macropinosome shrinkage. Nat Cell Biol 24:885-895 (2022). PubMed: 35590106