Anti-EDR1 / PHC1抗体[1F3F3] (ab175424)
![Western blot - Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424)](https://www.abcam.cn/ps/products/175/ab175424/Images/ab175424-202415-anti-edr1-phc1-antibody-1f3f3-western-blot.jpg "Western blot - Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424)")
Key features and details
- Mouse monoclonal [1F3F3] to EDR1 / PHC1
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
概述
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产品名称
Anti-EDR1 / PHC1抗体[1F3F3] -
描述
小鼠单克隆抗体[1F3F3] to EDR1 / PHC1 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cytmore details -
种属反应性
与反应: Human, Recombinant fragment
预测可用于: Mouse
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免疫原
Recombinant fragment corresponding to Human EDR1/ PHC1 aa 758-1004. Expressed in E. Coli.
Sequence:LKESEKPLQTGLPTGLTENQSGGPLGVDSPSAELDKKANLLKCEYCGKYA PAEQFRGSKRFCSMTCAKRYNVSCSHQFRLKRKKMKEFQEANYARVRRRG PRRSSSDIARAKIQGKCHRGQEDSSRGSDNSSYDEALSPTSPGPLSVRAG HGERDLGNPNTAPPTPELHGINPVFLSSNPSRWSVEEVYEFIASLQGCQE IAEEFRSQEIDGQALLLLKEEHLMSAMNIKLGPALKICAKINVLKET
Database link: P78364 -
阳性对照
- EDR1 / PHC1 recombinant protein; HEK293 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
0.5% protein stabilizer -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
1F3F3 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175424于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 106 kDa.
|
|
| Flow Cyt |
1/200 - 1/400.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 106 kDa. |
|
Flow Cyt
1/200 - 1/400. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. -
序列相似性
Contains 1 FCS-type zinc finger.
Contains 1 SAM (sterile alpha motif) domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1911 Human
- Entrez Gene: 13619 Mouse
- Omim: 602978 Human
- SwissProt: P78364 Human
- SwissProt: Q64028 Mouse
- Unigene: 305985 Human
- Unigene: 6822 Mouse
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别名
- Early development regulatory protein 1 antibody
- hPH1 antibody
- mPH1 antibody
see all
图片
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Western blot - Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424)All lanes : Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : EDR1/PHC1 (amino acids 758-1004)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 106 kDa -
![Western blot - Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424)](https://www.abcam.cn/ps/products/175/ab175424/Images/ab175424-202414-anti-edr1-phc1-antibody-1f3f3-western-blot.jpg)
Western blot - Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424)Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424) at 1/500 dilution + EDR1/PHC1 recombinant protein (amino acids 758-1004).
Predicted band size: 106 kDa -
![Flow Cytometry - Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424)](https://www.abcam.cn/ps/products/175/ab175424/Images/ab175424-202413-anti-edr1-phc1-antibody-1f3f3-flow-cytometry.jpg)
Flow Cytometry - Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424)Flow cytometric analysis of HEK293 cells labeling EDR1 / PHC1 with ab175424 at 1/200 dilution (green) and negative control (red).
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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