Anti-EDJ抗体(ab75107)
Key features and details
- Rabbit polyclonal to EDJ
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-EDJ抗体
参阅全部 EDJ 一抗 -
描述
兔多克隆抗体to EDJ -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
-
免疫原
Synthetic peptide corresponding to Human EDJ aa 31-80 (internal sequence).
Database link: Q9UBS4 -
阳性对照
- IHC-P: human heart tissue. WB: extracts from HeLa cells, HUVEC cells, COLO cells and MCF-7 cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75107于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 40 kDa.
|
|
| IHC-P |
1/50 - 1/100.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 40 kDa. |
|
IHC-P
1/50 - 1/100. |
靶标
-
功能
Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity. -
组织特异性
Widely expressed. -
序列相似性
Contains 1 J domain. -
翻译后修饰
Contains high-mannose Endo H-sensitive carbohydrates.
Cys-169, Cys-171, Cys-193 and Cys-196 form intramolecular disulfide bonds. The preferential partner for each Cys is not known.
Thr-188 was reported (PubMed:17525332) to be phosphorylated upon DNA damage by ATM or ATR; however as this position has been shown to be in the ER lumen, the in vivo relevance is not proven. -
细胞定位
Endoplasmic reticulum lumen. Associated with the ER membrane in a C-terminally epitope-tagged construct. - Information by UniProt
-
数据库链接
- Entrez Gene: 51726 Human
- Entrez Gene: 67838 Mouse
- Entrez Gene: 360734 Rat
- Omim: 611341 Human
- SwissProt: Q9UBS4 Human
- SwissProt: Q99KV1 Mouse
- SwissProt: Q6TUG0 Rat
- Unigene: 317192 Human
see all -
别名
- ABBP-2 antibody
- ABBP2 antibody
- APOBEC1 binding protein 2 antibody
see all
图片
-
Western blot - Anti-EDJ antibody (ab75107)All lanes : Anti-EDJ antibody (ab75107) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from HUVEC
cells
Lane 3 : extracts from COLO cells
Lane 4 : extracts from MCF-7 cells
Lane 5 : extracts from HeLa cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDJ antibody (ab75107)Immunohistochemical analysis of EDJ in paraffin embedded human heart tissue using 1/50 ab75107.
Left image: untreated sample.
Right image: sample treated with immunising peptide.
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab75107 被引用在 3 文献中.
- Cornec-Le Gall E et al. Monoallelic Mutations to DNAJB11 Cause Atypical Autosomal-Dominant Polycystic Kidney Disease. Am J Hum Genet 102:832-844 (2018). PubMed: 29706351
- Croner RS et al. Quantitative proteome profiling of lymph node-positive vs. -negative colorectal carcinomas pinpoints MX1 as a marker for lymph node metastasis. Int J Cancer 135:2878-86 (2014). PubMed: 24771638
- Xu W et al. Cigarette smoking exposure alters pebp1 DNA methylation and protein profile involved in MAPK signaling pathway in mice testis. Biol Reprod 89:142 (2013). PubMed: 24198121