Anti-EDJ抗体(ab224082)
Key features and details
- Rabbit polyclonal to EDJ
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-EDJ抗体
参阅全部 EDJ 一抗 -
描述
兔多克隆抗体to EDJ -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Dog, Orangutan
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免疫原
Recombinant fragment corresponding to Human EDJ aa 200 to the C-terminus.
Database link: Q9UBS4 -
阳性对照
- WB: HeLa, NIH/3T3 and NBT-II cell lysate. ICC/IF: U-2 OS cells. IHC-P: Human pancreas tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224082于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 40 kDa.
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 40 kDa. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity. -
组织特异性
Widely expressed. -
序列相似性
Contains 1 J domain. -
翻译后修饰
Contains high-mannose Endo H-sensitive carbohydrates.
Cys-169, Cys-171, Cys-193 and Cys-196 form intramolecular disulfide bonds. The preferential partner for each Cys is not known.
Thr-188 was reported (PubMed:17525332) to be phosphorylated upon DNA damage by ATM or ATR; however as this position has been shown to be in the ER lumen, the in vivo relevance is not proven. -
细胞定位
Endoplasmic reticulum lumen. Associated with the ER membrane in a C-terminally epitope-tagged construct. - Information by UniProt
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数据库链接
- Entrez Gene: 506316 Cow
- Entrez Gene: 478664 Dog
- Entrez Gene: 51726 Human
- Entrez Gene: 67838 Mouse
- Entrez Gene: 100462077 Orangutan
- Entrez Gene: 360734 Rat
- Omim: 611341 Human
- SwissProt: Q3ZBA6 Cow
see all -
别名
- ABBP-2 antibody
- ABBP2 antibody
- APOBEC1 binding protein 2 antibody
see all
图片
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Western blot - Anti-EDJ antibody (ab224082)Anti-EDJ antibody (ab224082) at 1/100 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 40 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-EDJ antibody (ab224082)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for EDJ (green) using ab224082 (4 µg/ml) in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDJ antibody (ab224082)Paraffin embedded human pancreas tissue stained for EDJ with ab224082 (1/200 dilution) in immunohistochemical analysis.
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Western blot - Anti-EDJ antibody (ab224082)All lanes : Anti-EDJ antibody (ab224082) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II (rat cell line) cell lysate
Predicted band size: 40 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab224082 被引用在 1 文献中.
- de Seny D et al. New Proteins Contributing to Immune Cell Infiltration and Pannus Formation of Synovial Membrane from Arthritis Diseases. Int J Mol Sci 23:N/A (2021). PubMed: 35008858