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AB214464

Anti-EDG8抗体

Anti-EDG8 antibody

5

(2 Reviews)

|

(1 Publication)

Rabbit Polyclonal EDG8 antibody. Suitable for IHC-P, Flow Cyt, WB and reacts with Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human S1PR5 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

查看别名

EDG8, S1PR5, Sphingosine 1-phosphate receptor 5, S1P receptor 5, S1P5, Endothelial differentiation G-protein-coupled receptor 8, Sphingosine 1-phosphate receptor Edg-8, S1P receptor Edg-8

3 Images
Western blot - Anti-EDG8 antibody (AB214464)
  • WB

Lab

Western blot - Anti-EDG8 antibody (AB214464)

All lanes:

Western blot - Anti-EDG8 antibody (ab214464) at 1/300 dilution

All lanes:

Mouse brain

Secondary

All lanes:

Conjugated secondary antibody at 1/20000 dilution

Predicted band size: 42 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG8 antibody (AB214464)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG8 antibody (AB214464)

Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded rat spleen tissue labeling EDG8 with ab214464 at 1/400 dilution, followed by conjugation to the secondary antibody and DAB staining.

Flow Cytometry - Anti-EDG8 antibody (AB214464)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-EDG8 antibody (AB214464)

Flow Cytometry of Mouse Spleen with ab214464 at 1/50.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat

应用

IHC-P, Flow Cyt, WB

applications

免疫原

Synthetic Peptide within Human S1PR5 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9H228

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100.00000 - 1/500.00000", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EDG8 also known as S1P5 is a receptor with a mass of approximately 41 kDa. It belongs to the G-protein coupled receptor family and specifically binds to sphingosine-1-phosphate (S1P). Researchers often find EDG8 expression in tissues such as the central nervous system spleen and placenta. It plays an important role in mediating cellular responses to extracellular signals through its interaction with ligands.
Biological function summary

S1P5 functions in cellular growth survival and migration. It is part of a receptor complex that mediates immune response and neurologic functions. This receptor transmits signals that regulate cellular shape and cytoskeletal architecture. S1P5 influences various processes such as lymphocyte trafficking and oligodendrocyte maturation supporting its role in immune and neurological systems.

Pathways

EDG8 engages in the sphingolipid signaling pathway and is also important in the regulation of the immune system. This receptor interacts with other proteins like G-protein subunits which transduce signals from extracellular factors and promote intracellular responses. The protein S1P5 links to the Akt and MAPK pathways which are important for cell survival and proliferation. These pathways help mediate cellular responses through signal transduction and protein activation.

S1P5 associates notably with multiple sclerosis and some types of cancer. Dysfunction in this receptor's signaling can disrupt normal cell function and immune regulation. S1P5's interactions with proteins like S1P1 and S1P3 further implicate its role in the progression of these diseases. Research continues to explore how modulating EDG8 can offer therapeutic strategies for managing these conditions effectively.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/0)alpha and G(12) subclass of heteromeric G-proteins (By similarity). May play a regulatory role in the transformation of radial glial cells into astrocytes and may affect proliferative activity of these cells.
See full target information S1PR5
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology research 8:1150-1162 PubMed32561537

2020

SRC-3 Functions as a Coactivator of T-bet by Regulating the Maturation and Antitumor Activity of Natural Killer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjia Hu,Yukai Lu,Yan Qi,Zihao Zhang,Song Wang,Yang Xu,Fang Chen,Yong Tang,Shilei Chen,Mo Chen,Changhong Du,Mingqiang Shen,Fengchao Wang,Yongping Su,Youcai Deng,Junping Wang
View all publications
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Abcam Product Promise

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详情请参阅我们的条款与条件。
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关联产品

Select an associated product type
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5

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