重组Anti-EDG3/S1P3抗体[EPR4541(2)] (ab126622)
![Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)](https://www.abcam.cn/ps/products/126/ab126622/Images/ab126622-140617-anti-edg3-s1p3-antibody-epr45412-western-blot.jpg "Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4541(2)] to EDG3/S1P3
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-EDG3/S1P3抗体[EPR4541(2)]
参阅全部 EDG3/S1P3 一抗 -
描述
兔单克隆抗体[EPR4541(2)] to EDG3/S1P3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human EDG3/S1P3 aa 300-150.
Database link: Q99500 -
阳性对照
- Human kidney tissue; Human heart, HepG2 and 293T cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 2.80 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4541(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-EDG3/S1P3 antibody [EPR4541(2)] - BSA and Azide free (ab248131)
- PE Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab318370)
- APC Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab318473)
- Alexa Fluor® 488 Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab318576)
- Alexa Fluor® 647 Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab318679)
- Alexa Fluor® 594 Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab318782)
- Alexa Fluor® 555 Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab318882)
- Alexa Fluor® 750 Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab321004)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126622于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
应用说明
Is unsuitable for Flow Cyt or IP.
靶标
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功能
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis. -
组织特异性
Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1903 Human
- Omim: 601965 Human
- SwissProt: Q99500 Human
- Unigene: 585118 Human
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别名
- EDG 3 antibody
- EDG3 antibody
- Endothelial differentiation G protein coupled receptor 3 antibody
see all
图片
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Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)All lanes : Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human heart tissue lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)](https://www.abcam.cn/ps/products/126/ab126622/Images/ab126622-140616-anti-edg3-s1p3-antibody-epr45412-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)ab126622, at a 1/50 dilution, staining EDG3/S1P3 in paraffin-embedded Human kidney tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![OI-RD Scanning - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)](https://www.abcam.cn/ps/products/126/ab126622/Images/ab126622-140615-anti-edg3-s1p3-antibody-epr45412.jpg)
OI-RD Scanning - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622) -
![Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)](https://www.abcam.cn/ps/products/126/ab126622/Images/ab126622-4-benefits-of-recombinant-antibodies.png)
Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab126622 被引用在 7 文献中.
- Lin Q et al. Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling. Exp Ther Med 23:3 (2022). PubMed: 34815755
- Lin Z et al. Targeting SPHK1/PBX1 Axis Induced Cell Cycle Arrest in Non-Small Cell Lung Cancer. Int J Mol Sci 23:N/A (2022). PubMed: 36361531
- Jia L et al. Triptolide Inhibits the Biological Processes of HUVECs and HepG2 Cells via the Serine Palmitoyltransferase Long Chain Base Subunit 2/Sphingosine-1-Phosphate Signaling Pathway. Dis Markers 2022:9119423 (2022). PubMed: 36438896
- Del Gaudio I et al. Circulating cord blood HDL-S1P complex preserves the integrity of the feto-placental vasculature. Biochim Biophys Acta Mol Cell Biol Lipids 1865:158632 (2020). PubMed: 31954174
- Palangi A et al. Differential expression of S1P receptor subtypes in human bladder transitional cell carcinoma. Clin Transl Oncol N/A:N/A (2019). PubMed: 30712233
- Shen Y et al. S1P/S1PR3 axis promotes aerobic glycolysis by YAP/c-MYC/PGAM1 axis in osteosarcoma. EBioMedicine N/A:N/A (2018). PubMed: 30587459
- Nicholas SE et al. Unravelling the interplay of sphingolipids and TGF-ß signaling in the human corneal stroma. PLoS One 12:e0182390 (2017). WB . PubMed: 28806736