Anti-EDF1抗体(ab174651)
Key features and details
- Rabbit polyclonal to EDF1
- Suitable for: IHC-Fr, IP, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-EDF1抗体 -
描述
兔多克隆抗体to EDF1 -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Cow
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免疫原
Synthetic peptide within Human EDF1 aa 98-148. The exact sequence is proprietary. NP_003783.1.
Sequence:KINEKPQVIADYESGRAIPNNQVLGKIERAIGLKLRGKDIGKPIEKGPRA K
Database link: O60869 -
阳性对照
- WB: HEK293T, HeLa, and Jurkat whole cell lysates. IP: HEK293T cells. IHC-Fr: Human breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab174651 was affinity purified using an epitope specific to EDF1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174651于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
1/500 - 1/2000.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 16 kDa.
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| 说明 |
|---|
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IHC-Fr
1/500 - 1/2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 16 kDa. |
靶标
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功能
Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism. -
组织特异性
Expressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues. -
序列相似性
Contains 1 HTH cro/C1-type DNA-binding domain. -
发展阶段
Expressed in fetal tissues. More abundant in kidney. -
结构域
The IQ motif, which is involved in calmodulin binding, overlaps with the binding domain for nuclear receptors and transcription factors. Its phosphorylation probably allows a switch between the two activities of the protein. -
翻译后修饰
Phosphorylated (by PKA and PKC). -
细胞定位
Cytoplasm. Nucleus. Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin. - Information by UniProt
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数据库链接
- Entrez Gene: 515380 Cow
- Entrez Gene: 8721 Human
- Omim: 605107 Human
- SwissProt: Q3T0V7 Cow
- SwissProt: O60869 Human
- Unigene: 174050 Human
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别名
- CAP19 antibody
- EDF-1 antibody
- edf1 antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Anti-EDF1 antibody (ab174651)
Immunohistochemical analysis of frozen section of Human breast carcinoma tissue with ab174651 at 1:1,000 (1µg/ml).
Detection: DAB
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Western blot - Anti-EDF1 antibody (ab174651)All lanes : Anti-EDF1 antibody (ab174651) at 0.100000001490116 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 16 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-EDF1 antibody (ab174651)Detection of EDF1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab174651 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab174651 was used at 1 µg/ml. Lane 2 represents control IgG IP.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab174651 被引用在 4 文献中.
- Ryder L et al. Nitric oxide-induced ribosome collision activates ribosomal surveillance mechanisms. Cell Death Dis 14:467 (2023). PubMed: 37495584
- Snieckute G et al. Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response. Cell Metab 34:2036-2046.e8 (2022). PubMed: 36384144
- Sinha NK et al. EDF1 coordinates cellular responses to ribosome collisions. Elife 9:N/A (2020). PubMed: 32744497
- Nishioka K et al. Mbf1 ensures Polycomb silencing by protecting E(z) mRNA from degradation by Pacman. Development 145:N/A (2018). PubMed: 29523653