Anti-EDD抗体(ab175810)
Key features and details
- Rabbit polyclonal to EDD
- Suitable for: IP, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-EDD抗体
参阅全部 EDD 一抗 -
描述
兔多克隆抗体to EDD -
宿主
Rabbit -
经测试应用
适用于: IP, WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human EDD aa 1900-2230.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175810于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 309 kDa.
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| ICC/IF |
1/1000.
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| IHC-P |
1/1000.
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| 说明 |
|---|
|
IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 309 kDa. |
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ICC/IF
1/1000. |
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IHC-P
1/1000. |
靶标
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功能
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. -
组织特异性
Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 PABC domain.
Contains 1 UBR-type zinc finger. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 51366 Human
- Entrez Gene: 70790 Mouse
- Entrez Gene: 117060 Rat
- Omim: 608413 Human
- SwissProt: O95071 Human
- SwissProt: Q80TP3 Mouse
- SwissProt: Q62671 Rat
- Unigene: 492445 Human
see all -
别名
- 4432411E13Rik antibody
- AW549941 antibody
- C77315 antibody
see all
图片
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Western blot - Anti-EDD antibody (ab175810)All lanes : Anti-EDD antibody (ab175810)
Lane 1 : Control si
Lane 2 : UBR5 si
Lane 3 : NIH 3T3 cell lysate at 40 µg
Lane 4 : PC12 cell lysate at 40 µg
Predicted band size: 309 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-EDD antibody (ab175810)Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling EDD with ab175810 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Top - EDD, Middle - DAPI, Bottom - Merge.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab175810)Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling EDD with ab175810 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9. Top - 4X, Middle - 10X, Bottom - 20X.
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Immunoprecipitation - Anti-EDD antibody (ab175810)Immunoprecipitation analysis of U2OS cells labelling EED with ab175810. Protein A beads were crosslinked to the serum.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab175810 被引用在 2 文献中.
- Zhao X et al. Targeted therapy for cisplatin-resistant lung cancer via aptamer-guided nano-zinc carriers containing USP14 siRNA. MedComm (2020) 4:e237 (2023). PubMed: 37035133
- Wu Q et al. UBR5 promotes migration and invasion of glioma cells by regulating the ECRG4/NF-ҡB pathway. J Biosci 47:N/A (2022). IHC-P ; Mouse . PubMed: 36226349