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AB21288

Anti-ECSIT抗体

Anti-ECSIT antibody

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(8 Publications)

Rabbit Polyclonal ECSIT antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human ECSIT.

查看别名

Protein SITPEC, ECSIT

1 Images
Western blot - Anti-ECSIT antibody (AB21288)
  • WB

Unknown

Western blot - Anti-ECSIT antibody (AB21288)

Lane 1:

Western blot - Anti-ECSIT antibody (ab21288) at 0.5 µg/mL

Lane 2:

Western blot - Anti-ECSIT antibody (ab21288) at 1 µg/mL

Lane 3:

Western blot - Anti-ECSIT antibody (ab21288) at 2 µg/mL

All lanes:

human heart lysate

Predicted band size: 49 kDa

Observed band size: 42 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human, Mouse

应用

IHC-P, IP, WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human ECSIT. The exact immunogen used to generate this antibody is proprietary information.

Q9BQ95

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
纯化说明
Ion exchange chromatography.
存储溶液
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ECSIT also known as Evolutionarily Conserved Signaling Intermediate in Toll pathways is a mitochondrial protein involved in various cellular processes. It has a molecular mass of approximately 52 kDa and is prominently expressed in tissues with high metabolic activity such as the heart and skeletal muscles. ECSIT interacts with the mitochondrial membrane and is integral for mitochondrial function and biogenesis. Understanding the expression and function of ECSIT can provide insight into its role in cellular energy homeostasis.
Biological function summary

ECSIT influences several important cellular processes through its participation in the assembly of the mitochondrial complex I. It is part of a protein complex that includes NDUFAF1 and acts as a scaffold protein facilitating the proper assembly of complex I. ECSIT involvement is important for the maintenance of cellular respiration and energy production. Its role in complex I assembly highlights its importance in regulating mitochondrial activity and function.

Pathways

ECSIT acts in both mitochondrial biogenesis and the toll-like receptor (TLR) signaling pathway. In the TLR pathway ECSIT mediates interactions between TRAF6 and TAK1 contributing to the regulation of immune responses. It is an essential component linking mitochondrial function with immune signaling demonstrating its multi-functional capability. These pathways highlight the intricate network where ECSIT collaborates with proteins like TRAF6 and TAK1 to ensure proper immune response and energy metabolism.

ECSIT has associations with leigh syndrome and aggressive lung cancer. Its dysfunction in mitochondrial biogenesis can contribute to Leigh syndrome a severe neurological disorder. In lung cancer ECSIT involvement in TLR signaling affects cell proliferation and survival making it a protein of interest in cancer research. ECSIT's role in these conditions can involve interactions with mitochondrial proteins and signaling molecules like TRAF6 further illustrating its impact on human health.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Adapter protein that plays a role in different signaling pathways including TLRs and IL-1 pathways or innate antiviral induction signaling. Plays a role in the activation of NF-kappa-B by forming a signal complex with TRAF6 and TAK1/MAP3K7 to activate TAK1/MAP3K7 leading to activation of IKKs (PubMed : 25355951, PubMed : 31281713). Once ubiquitinated, interacts with the dissociated RELA and NFKB1 proteins and translocates to the nucleus where it induces NF-kappa-B-dependent gene expression (PubMed : 25355951). Plays a role in innate antiviral immune response by bridging the pattern recognition receptors RIGI and MDA5/IFIT1 to the MAVS complex at the mitochondrion (PubMed : 25228397). Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity).. As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
See full target information ECSIT
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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 14:1110369 PubMed37152948

2023

ECSIT is essential for RANKL-induced stimulation of mitochondria in osteoclasts and a target for the anti-osteoclastogenic effects of estrogens.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana Marques-Carvalho,Vilma A Sardão,Ha-Neui Kim,Maria Almeida

iScience 24:103047 PubMed34553133

2021

Synthetic VSMCs induce BBB disruption mediated by MYPT1 in ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Hailan Meng,Lizhen Fan,Cun-Jin Zhang,Liwen Zhu,Pinyi Liu,Jian Chen,Xinyu Bao,Zhijun Pu,Min-Sheng Zhu,Yun Xu

Journal of immunology (Baltimore, Md. : 1950) 207:1627-1640 PubMed34433619

2021

Metabolic Adaptation of Macrophages as Mechanism of Defense against Crystalline Silica.

Applications

Unspecified application

Species

Unspecified reactive species

Antonella Marrocco,Krystin Frawley,Linda L Pearce,James Peterson,James P O'Brien,Steven J Mullett,Stacy G Wendell,Claudette M St Croix,Steven E Mischler,Luis A Ortiz

Scientific reports 10:11183 PubMed32636430

2020

MTFMT deficiency correlates with reduced mitochondrial integrity and enhanced host susceptibility to intracellular infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Hwa Seo,Cheol-Sang Hwang,Joo-Yeon Yoo

Frontiers in immunology 10:2203 PubMed31620128

2019

CRBN Is a Negative Regulator of Bactericidal Activity and Autophagy Activation Through Inhibiting the Ubiquitination of ECSIT and BECN1.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Jeong Kim,Yoon Min,Jae-Hyuck Shim,Eunyoung Chun,Ki-Young Lee

Molecular and cellular biology 34:847-61 PubMed24344204

2013

TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex.

Applications

Unspecified application

Species

Unspecified reactive species

Virginia Guarani,Joao Paulo,Bo Zhai,Edward L Huttlin,Steven P Gygi,J Wade Harper

Genome research 21:364-76 PubMed21163940

2010

Interactome mapping suggests new mechanistic details underlying Alzheimer's disease.

Applications

IP, WB, ICC/IF

Species

Human, Human, Human

Montserrat Soler-López,Andreas Zanzoni,Ricart Lluís,Ulrich Stelzl,Patrick Aloy

Genes & development 21:615-24 PubMed17344420

2007

Cytosolic signaling protein Ecsit also localizes to mitochondria where it interacts with chaperone NDUFAF1 and functions in complex I assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Rutger O Vogel,Rolf J R J Janssen,Mariël A M van den Brand,Cindy E J Dieteren,Sjoerd Verkaart,Werner J H Koopman,Peter H G M Willems,Wendy Pluk,Lambert P W J van den Heuvel,Jan A M Smeitink,Leo G J Nijtmans
View all publications
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