Anti-EBI3抗体(ab83896)
Key features and details
- Rabbit polyclonal to EBI3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-EBI3抗体
参阅全部 EBI3 一抗 -
描述
兔多克隆抗体to EBI3 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Rat, Human -
免疫原
16 amino acid synthetic peptide corresponding to an internal sequence of Human EBI3
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab83896于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
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| IHC-P |
Use a concentration of 2.5 µg/ml.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa). |
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IHC-P
Use a concentration of 2.5 µg/ml. |
靶标
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功能
Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines. -
序列相似性
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 2 fibronectin type-III domains. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 10148 Human
- Entrez Gene: 680609 Rat
- Omim: 605816 Human
- SwissProt: Q14213 Human
- Unigene: 501452 Human
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别名
- cytokine receptor antibody
- EBI3 antibody
- EBV induced gene 3 protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody (ab83896)ab83896 at 2.5µg/ml staining EBI3 in human spleen tissue.
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Western blot - Anti-EBI3 antibody (ab83896)Anti-EBI3 antibody (ab83896) at 2 µg/ml + Rat spleen tissue lysate at 15 µg
Secondary
Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Diluting buffer: 5% NFDM/TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-EBI3 antibody (ab83896)Immunofluorescence of EBI3 in Human Spleen cells using ab83896 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab83896 被引用在 6 文献中.
- Li X et al. JNK/c-Jun pathway activation is essential for HBx-induced IL-35 elevation to promote persistent HBV infection. J Clin Lab Anal 37:e24860 (2023). PubMed: 36916737
- Zhu J et al. Interleukin-35 promotes progression of prostate cancer and inhibits anti-tumour immunity. Cancer Cell Int 20:487 (2020). PubMed: 33041668
- Shu C et al. Metformin exhibits its therapeutic effect in the treatment of pre-eclampsia via modulating the Met/H19/miR-148a-5p/P28 and Met/H19/miR-216-3p/EBI3 signaling pathways. Int Immunopharmacol 74:105693 (2019). PubMed: 31203154
- Zhu J et al. Interleukin-35 is associated with the tumorigenesis and progression of prostate cancer. Oncol Lett 17:5094-5102 (2019). PubMed: 31186721
- Umezawa N et al. Interleukin-23 as a therapeutic target for inflammatory myopathy. Sci Rep 8:5498 (2018). PubMed: 29615652
- Al-Sammak F et al. Gastric Epithelial Expression of IL-12 Cytokine Family in Helicobacter pylori Infection in Human: Is it Head or Tail of the Coin? PLoS One 8:e75192 (2013). IHC-P ; Human . PubMed: 24069393