Anti-EB3抗体[KT36] (ab53360)

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to confirm you that ab53360 has not been tested for use in mouse samples and therefore is not covered by our 6 month guarantee.

Reviewing this case, I would like to offer some suggestions to help optimise the results from ab53360.

1.) Some epitopes are very sensitive to methanol as it can disrupt epitope structure. Can try acetone instead for permeabilization if required or use 4% PFA and a permeabilisation agent.

2.) I suggest to use 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions.

3.) I Since the secondary antibody alone give high background, I strongly recommend to use a duifferent antibody. Since mouse and rat are very closely related, I suggest to use a secondary antibody that is pre-absorped against cross-reactivity to rat.

I would also appreciate if you can confirm some further details:

Please send an image of your results, this will help us to provide suitable tips.

I hope this information is helpful, thank you for your cooperation.

Thank you for your reply.

Thats no problem. However, if you would like to participate in this scheme please do let us know prior to purchasing the antibody. I have generated the discount code as requested:

Discount code: xxxxxxx

Expiration date: 19th of December 2012

This code will give you: 1 freeprimary antibody or the value of ab53360off any product in our catalogue before the expiration date above. To redeem this offer, please submit an Abreview formouse and/or ratand include this code in the “Additional Comments” section so we know the Abreview is for this promotion.

Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site:

https://www.abcam.com/Abreviews

We publish both positive and negative Abreviews on our datasheets so please submit the results of your tests, whatever you find. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here:

https://www.abcam.com/collaborationdiscount

Thank you for your reply.

The immunogen used to raise the anti-EB3 antibody [KT36] (ab53360) was of the recombinantly expressed full length human EB3 protein. This shares 99% homology with the rat and mouse protein. We would therefore predict that the antibody would cross react. However, as wehave not tested this ourselves as yet we would not be able to guarantee this.

However, if you would like to purchase ab53360 and use it with yourmouse and ratsamples, we can offer you a testing discount. This offer means that if you purchase ab53360 as normal, test the antibody with yoursamples and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab53360 off any product). More information on this offer can be found here:

www.abcam.com/collaborationdiscount

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab53360.

Alternatively, you may want to have a look at the rabbit polyclonal ab13859 which we have shown is able to detect the human, rat and mouse protein.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Thank you for contacting us and your interest in our products.

As yet, we have not tested the Anti-EB3 antibody [KT36] (ab53360) in any rodent species. However, this does not mean that the antibody would not work. As the immunogen used to raise the antibody, full length human EB3 fused to a tag, shares 99% homology with the mouse and rat protein, we would expect the antibody to be able to detect these proteins. As we have not tested it we would not be able to guarantee it.

If you can describe the experiment you are hoping to perform (which type of samples and application)I can see if I would recommend trying this antibody or if we have any other antibodies against this target which may be more suitable.

I look forward to receiving your reply.