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AB201327

Anti-Eagle抗体[8B11DH10]

Anti-Eagle antibody [8B11DH10]

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(1 Publication)

Mouse Monoclonal Eagle antibody. Suitable for IHC (PFA fixed) and reacts with Drosophila melanogaster samples. Cited in 1 publication.

查看别名

egon, NR0A3, spry, CG7383, eg, Protein embryonic gonad, Nuclear receptor subfamily 0 group A member 3, Protein eagle, Protein spready

1 Images
Immunohistochemistry (PFA fixed) - Anti-Eagle antibody [8B11DH10] (AB201327)
  • IHC (PFA fixed)

Supplier Data

Immunohistochemistry (PFA fixed) - Anti-Eagle antibody [8B11DH10] (AB201327)

Immunohistochemistry analysis in neuroblast nuclei from Drosophila stage 15 embryos. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum.The Eagle antibody (ab201327) was used at 3.8 μg/mL, overnight at 4°C. The secondary antibody used was Alexa488 conjugated goat anti-mouse at 1/400.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

8B11DH10

亚型

IgG1

轻链类型

kappa

不含载体蛋白

No

反应种属

Drosophila melanogaster

应用

IHC (PFA fixed)

applications

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCPFAfixed" : {"fullname" : "Immunohistochemistry (PFA fixed)", "shortname":"IHC (PFA fixed)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Drosophila melanogaster": { "IHCPFAfixed-species-checked": "testedAndGuaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "<p></p>" } } }
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产品详情

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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性能和储存信息

形式
Liquid
纯化说明
Purity: 95%
存储溶液
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99% HEPES buffered saline
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Eagle also known as ELK3 or Net is a nuclear protein that weighs approximately 44 kDa. It is a member of the ETS domain transcription factor family. Eagle primarily localizes in the nucleus and is widely expressed in various cell types with higher concentrations observed in the spleen and thymus. The protein shows a significant affinity for binding to DNA sequences and influences the transcriptional regulation of genes involved in cellular proliferation and differentiation.
Biological function summary

Eagle plays a significant role in the regulation of gene expression. It functions as part of a multiprotein complex involving other ETS domain family members. Eagle can act as both a transcriptional activator and repressor depending on the cellular context. Its regulatory activities are important in the processes of vascular development and maintenance of normal cellular function.

Pathways

Eagle is actively involved in the Ras-MAPK signaling pathway. This pathway is important for transmitting signals from the cell surface to the nucleus controlling cell cycle progression and modulating differentiation responses. Eagle interacts closely with other proteins in this pathway such as MEK and ERK influencing the transcriptional regulation downstream of the signaling cascade. Eagle's participation in this pathway reflects its ability to affect various cellular outcomes through modulating gene expression.

Eagle has been associated with several types of cancer including breast and colorectal cancer. Its dysregulation affects the transcriptional control of genes linked to uncontrolled cell proliferation. Eagle's interaction with the protein c-Fos in these cancers contributes to altered signaling pathways that favor oncogenesis. Understanding Eagle's role in these diseases highlights its potential as a target for therapeutic intervention in cancer research.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

May play a critical role in development of the progeny of eagle-positive neuroblasts.
See full target information eg
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 23:e3002881 PubMed40163536

2025

The Hunchback transcription factor determines interneuron molecular identity, morphology, and presynapse targeting in the Drosophila NB5-2 lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Heather Q Pollington,Chris Q Doe
View all publications
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