Anti-EAAT2抗体(ab41621)
Key features and details
- Rabbit polyclonal to EAAT2
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-EAAT2抗体
参阅全部 EAAT2 一抗 -
描述
兔多克隆抗体to EAAT2 -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Synthetic peptide within Rat EAAT2 aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P31596 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41621于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 10 µg/ml.
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| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
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| 说明 |
|---|
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ICC
Use a concentration of 10 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). |
靶标
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功能
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. -
序列相似性
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily. -
翻译后修饰
Glycosylated. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 20511 Mouse
- Entrez Gene: 29482 Rat
- SwissProt: P43006 Mouse
- SwissProt: P31596 Rat
- Unigene: 267547 Mouse
- Unigene: 371582 Mouse
- Unigene: 10240 Rat
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别名
- EAA2_HUMAN antibody
- EAAT2 antibody
- Excitatory amino acid transporter 2 antibody
see all
图片
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Immunocytochemistry - Anti-EAAT2 antibody (ab41621)
ab41621 staining EAAT2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab41621 at 10µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-EAAT2 antibody (ab41621)All lanes : Anti-EAAT2 antibody (ab41621) at 1 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 62 kDa -
Immunocytochemistry - Anti-EAAT2 antibody (ab41621)This image is courtesy of an anonymous Abreview.Methanol fixed human astrocytes (differentiated from H9-derived neuronal) cells labeling EAAT2 using ab41621 at a 1/250 dilution, 1 hr, 22°C (green) followed by an Alexa Fluor® 488 secondary antibody (1/1000 dilution), in ICC/IF.
Cells were blocked in 5% BSA for 30 mins, 22°C.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (85)
ab41621 被引用在 85 文献中.
- Huang YJ et al. Astrocytic aryl hydrocarbon receptor mediates chronic kidney disease-associated mental disorders involving GLT1 hypofunction and neuronal activity enhancement in the mouse brain. Glia 71:1057-1080 (2023). PubMed: 36573349
- Fernández-Rodríguez S et al. N-Acetylcysteine normalizes brain oxidative stress and neuroinflammation observed after protracted ethanol abstinence: a preclinical study in long-term ethanol-experienced male rats. Psychopharmacology (Berl) 240:725-738 (2023). PubMed: 36708386
- Thongrong S et al. Pinostrobin alleviates chronic restraint stress‑induced cognitive impairment by modulating oxidative stress and the function of astrocytes in the hippocampus of rats. Biomed Rep 18:20 (2023). PubMed: 36798091
- Balkaya M et al. Conditional deletion of LRRC8A in the brain reduces stroke damage independently of swelling-activated glutamate release. iScience 26:106669 (2023). PubMed: 37182109
- González-Alfonso WL et al. Chronic exposure to inorganic arsenic and fluoride induces redox imbalance, inhibits the transsulfuration pathway, and alters glutamate receptor expression in the brain, resulting in memory impairment in adult male mouse offspring. Arch Toxicol 97:2371-2383 (2023). PubMed: 37482551