AB41621

Anti-EAAT2 抗体

Name: EAAT2抗体 (ab41621)| Abcam中文官网
Brand: Abcam Limited
SKU: AB41621
Price: 4218 CNY
Availability: InStock
Rating: 4 (6 reviews)

Anti-EAAT2 antibody

(6 Reviews)

(117 Publications)

Anti-EAAT2 antibody (ab41621) is a rabbit polyclonal antibody detecting EAAT2 in Western Blot. Suitable for Mouse, Rat.

- Over 80 publications
- Trusted since 2007

查看别名

Eaat2, Glt1, Slc1a2, Excitatory amino acid transporter 2, GLT-1, Sodium-dependent glutamate/aspartate transporter 2, Solute carrier family 1 member 2

3 Images

Project

Western blot - Anti-EAAT2 antibody (AB41621)

All lanes:

Western blot - Anti-EAAT2 antibody (ab41621) at 1 µg/mL

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

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AbReview66659****

Immunocytochemistry - Anti-EAAT2 antibody (AB41621)

Methanol fixed human astrocytes (differentiated from H9-derived neuronal) cells labeling EAAT2 using ab41621 at a 1/250 dilution, 1 hr, 22°C (green) followed by an Alexa Fluor^® 488 secondary antibody (1/1000 dilution), in ICC/IF.

Cells were blocked in 5% BSA for 30 mins, 22°C.

This image is courtesy of an anonymous Abreview.

Lab

Immunocytochemistry - Anti-EAAT2 antibody (AB41621)

ab41621 staining EAAT2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab41621 at 10μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat

应用

WB, ICC

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-EAAT2 antibody (ab41621) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Mouse, Rat samples.

What is the molecular weight of EAAT2?
Anti-EAAT2 (ab41621) specifically detects a band for EAAT2 (UniProt: P31596) at a molecular weight of 62kDa.

Trusted by the scientific community
Anti-EAAT2 (ab41621) was first used in a scientific publication in 2007 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists
Anti-EAAT2 (ab41621) has over 5 independent reviews from customers.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EAAT2 also known as excitatory amino acid transporter 2 or GLT-1 is a protein responsible for reuptake of glutamate from the synaptic cleft into glial cells preventing excitotoxicity. The protein has a mass of approximately 73 kDa and prominently expresses in the central nervous system particularly in astrocytes. By regulating extracellular glutamate levels EAAT2 helps maintain neurotransmitter balance critical for healthy neuron function.

Biological function summary

EAAT2 plays an important role in maintaining synaptic transmission and preventing overexcitation that can lead to neuronal damage. It is part of a transporter complex responsible for the movement of glutamate across the cell membrane working together with ions like sodium and potassium. Due to its significant role in the regulation of neurotransmitter levels in the brain EAAT2 is considered an important player in neural communication processes.

Pathways

EAAT2 is integral to the glutamatergic signaling pathway where its activity supports glutamate recycling and homeostasis. This pathway is important for normal cognitive functions such as learning and memory. EAAT2 works closely with other proteins such as EAAT1 in efforts to regulate glutamate concentrations in the extracellular space impacting synaptic strength and plasticity in the nervous system.

Malfunctions of EAAT2 are associated with conditions like amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. In ALS EAAT2 dysfunction leads to accumulation of neurotoxic levels of glutamate causing motor neuron death. Similarly in Alzheimer’s disease impaired regulation of EAAT2 contributes to neurodegeneration through a related increase in neuronal excitotoxicity. Both conditions emphasize the critical importance of EAAT2 in neuroprotection and maintenance of normal brain function.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed : 7557442, PubMed : 7698742, PubMed : 9373176). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate (PubMed : 9180080).

See full target information Slc1a2

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文献 (117)

Recent publications for all applications. Explore the full list and refine your search

IBRO neuroscience reports 19:54-61 PubMed40535312

2025

Brain pericytes upregulate glutamate uptake by astrocytes through sodium-dependent transporter.

Applications

Unspecified application

Species

Unspecified reactive species

Kenta Sakai,Fuyuko Takata,Takuro Iwao,Miho Yasunaga,Gaku Yamanaka,Yasufumi Kataoka,Shinya Dohgu

Glia 73:1090-1106 PubMed40103346

2025

Distinguishing Neuromyelitis Optica Spectrum Disorders Subtypes: A Study on AQP4 and C3d Epitope Expression in Cytokine-Primed Human Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Marlen Alisch,Franziska Foersterling,Dario Zocholl,Bakhrom Muinjonov,Patrick Schindler,Ankelien Duchnow,Carolin Otto,Klemens Ruprecht,Tanja Schmitz-Hübsch,Sven Jarius,Friedemann Paul,Volker Siffrin

Acta neuropathologica communications 12:166 PubMed39434170

2024

PS1/gamma-secretase acts as rogue chaperone of glutamate transporter EAAT2/GLT-1 in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Perrin,Lauren C Anderson,Shane P C Mitchell,Priyanka Sinha,Yuliia Turchyna,Masato Maesako,Mei C Q Houser,Can Zhang,Steven L Wagner,Rudolph E Tanzi,Oksana Berezovska

Cells 13: PubMed39404364

2024

Glutamate Transporter 1 as a Novel Negative Regulator of Amyloid β.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka Sinha,Yuliia Turchyna,Shane Patrick Clancy Mitchell,Michael Sadek,Gokce Armagan,Florian Perrin,Masato Maesako,Oksana Berezovska

Glia 72:2022-2037 PubMed39046219

2024

Dysfunctional astrocyte glutamate uptake in the hypothalamic paraventricular nucleus contributes to visceral pain and anxiety-like behavior in mice with chronic pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Luo,Xiaojun Hu,Xin Li,Fan Lei,Ping Liao,Limei Yi,Xia Zhang,Bin Zhou,Ruotian Jiang

International journal of molecular sciences 25: PubMed39000602

2024

Inhibition of Indoleamine 2,3-Dioxygenase Exerts Antidepressant-like Effects through Distinct Pathways in Prelimbic and Infralimbic Cortices in Rats under Intracerebroventricular Injection with Streptozotocin.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Qin,Xiao Hu,Hui-Ling Zhao,Nurhumar Kurban,Xi Chen,Jing-Kun Yi,Yuan Zhang,Su-Ying Cui,Yong-He Zhang

Neuroscience 552:89-99 PubMed38909675

2024

Effects of MC-100093 on Ethanol Drinking and the Expression of Astrocytic Glutamate Transporters in the Mesocorticolimbic Brain Regions of Male and Female Alcohol-Preferring Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Alotaibi,Shelby Travaglianti,Woonyen Wong,Magid Abou-Gharbia,Wayne Childers,Youssef Sari

Nature communications 15:4695 PubMed38824138

2024

Co-aggregation with Apolipoprotein E modulates the function of Amyloid-β in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Zengjie Xia,Emily E Prescott,Agnieszka Urbanek,Hollie E Wareing,Marianne C King,Anna Olerinyova,Helen Dakin,Tom Leah,Katy A Barnes,Martyna M Matuszyk,Eleni Dimou,Eric Hidari,Yu P Zhang,Jeff Y L Lam,John S H Danial,Michael R Strickland,Hong Jiang,Peter Thornton,Damian C Crowther,Sohvi Ohtonen,Mireia Gómez-Budia,Simon M Bell,Laura Ferraiuolo,Heather Mortiboys,Adrian Higginbottom,Stephen B Wharton,David M Holtzman,Tarja Malm,Rohan T Ranasinghe,David Klenerman,Suman De

Frontiers in cellular neuroscience 18:1347535 PubMed38650656

2024

Impaired astrocytic synaptic function by peripheral cholesterol metabolite 27-hydroxycholesterol.

Applications

Unspecified application

Species

Unspecified reactive species

Fokion Spanos,Gorka Gerenu,Julen Goikolea,María Latorre-Leal,Hugo Balleza-Tapia,Karen Gomez,Laura Álvarez-Jiménez,Antonio Piras,Marta Gómez-Galán,André Fisahn,Angel Cedazo-Minguez,Silvia Maioli,Raúl Loera-Valencia

Neuron 112:1978-1996.e6 PubMed38599212

2024

Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Saptarshi Biswas,Sanjid Shahriar,Galina Bachay,Panos Arvanitis,Danny Jamoul,William J Brunken,Dritan Agalliu

View all publications

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