Anti-E4F1抗体(ab70618)
Key features and details
- Rabbit polyclonal to E4F1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-E4F1抗体
参阅全部 E4F1 一抗 -
描述
兔多克隆抗体to E4F1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan, Bat
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免疫原
Synthetic peptide corresponding to Human E4F1 aa 700-800 (C terminal). Synthetic peptide corresponding to a region between residue 725 and the C terminus (residue 784) of human E4F1
Database link: Q66K89 -
阳性对照
- HeLa, 293T and MCF7 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab70618 was affinity purified using an epitope specific to E4F1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70618于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| IP |
| 说明 |
|---|
WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 84 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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相关性
E4F1 is a zinc finger transcription factor that inhibits cell proliferation. E4F1 interacts with Bmi1 in hematopoietic cells and is important for the regulation of stem cell proliferation. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 454356 Chimpanzee
- Entrez Gene: 515997 Cow
- Entrez Gene: 1877 Human
- Entrez Gene: 13560 Mouse
- Entrez Gene: 681359 Rat
- Omim: 603022 Human
- SwissProt: Q66K89 Human
- SwissProt: Q8CCE9 Mouse
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别名
- E4F antibody
- E4F transcription factor 1 antibody
- E4F transcription factor 1, isoform CRA_b antibody
see all
图片
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Western blot - Anti-E4F1 antibody (ab70618)All lanes : Anti-E4F1 antibody (ab70618) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 84 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Immunoprecipitation - Anti-E4F1 antibody (ab70618)Detection of Human E4F1 by Western Blot of Immunprecipitate. Lane 1: ab70618 at 1µg/ml staining E4F1 in MCF7 whole cell lysates immunoprecipitated using ab70618 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 3 minutes.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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