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AB137500

Anti-E3 SUMO-protein ligase PIAS4/PIASy抗体

Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody

0

(1 Review)

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(4 Publications)

Rabbit Polyclonal E3 SUMO-protein ligase PIAS4/PIASy antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PIAS4 aa 400-500.

查看别名

PIASG, PIAS4, E3 SUMO-protein ligase PIAS4, PIASy, Protein inhibitor of activated STAT protein 4, Protein inhibitor of activated STAT protein gamma, PIAS-gamma

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)

Confocal immunofluorescence analysis analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell labelling E3 SUMO-protein ligase PIAS4 with ab137500 (green) at a 1/500 dilution. Alpha-tubulin filaments were labeled with an Alpha-tubulin antibody (red) at a 1/2000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft tissue staining E3 SUMO-protein ligase PIAS4 with ab137500 at a 1/100 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue staining E3 SUMO-protein ligase PIAS4 with ab137500 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)
  • WB

Supplier Data

Western blot - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)

10% SDS-PAGE

All lanes:

Western blot - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab137500) at 1/5000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

PIAS4-transfected HEK-293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 56 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

ICC/IF, IHC-P, WB

applications

免疫原

Synthetic Peptide within Human PIAS4 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

Q8N2W9

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替代产品

Primary Antibodies

AB58416

Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB58416)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB32058

Anti-Sumo 1 antibody [Y299] - ChIP Grade

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (AB32058)

  • 4
  • 11
Primary Antibodies

AB115777

Anti-beta Actin antibody [SP124] - Cytoskeleton Marker

20ul selling size|RabMAb|Lab Essentials|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (AB115777)

  • 4
  • 1
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB75854

Anti-UBE2I / UBC9 antibody [EP2938Y]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-UBE2I / UBC9 antibody [EP2938Y] (AB75854)

  • 4
  • 8
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The E3 SUMO-protein ligase PIAS4 also known as PIASy functions as an E3-type small ubiquitin-like modifier (SUMO) ligase. This protein plays a role in SUMOylation a post-translational modification process where it catalyzes the transfer of SUMO from E2 conjugating enzyme to target substrates. PIAS4 has a mass of approximately 57 kDa and is expressed in various tissues including the brain heart and skeletal muscle. It shows localization in the nucleus reflecting its role in regulating nuclear processes.
Biological function summary

This protein modulates transcription by interacting with and modifying transcription factors through SUMOylation affecting their stability and activity. PIAS4 participates in multiprotein complexes which can influence gene expression and cellular responses to stress. Its role in DNA repair processes is significant as it SUMOylates proteins involved in homologous recombination and DNA damage response pathways aiding in maintaining genomic integrity.

Pathways

PIAS4 plays a role in the regulation of DNA damage repair and the STAT signaling pathway. It cooperates with proteins like BRCA1 and RAD51 in DNA repair ensuring the correct repair of double-strand breaks. In the STAT signaling pathway PIAS4 influences STAT transcription factors impacting various cellular activities including growth and apoptosis. This modulation links PIAS4 to cellular responses relevant to growth and stress.

PIAS4 has connections to cancer and neurodegenerative diseases. Mutations or altered expression of PIAS4 can disrupt DNA repair processes leading to an increased susceptibility to cancer due to accumulated genomic instability. PIAS4 also interacts with proteins like p53 in pathways associated with cancer development. Additionally its involvement in stress response mechanisms could link it to neurodegenerative diseases possibly through affecting neuronal cell survival and function.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor (PubMed : 12511558, PubMed : 12631292, PubMed : 12727872, PubMed : 15831457, PubMed : 15976810, PubMed : 22508508, PubMed : 32832608). Mediates sumoylation of ALKBH5, AXIN1, CEBPA, KLF8, GATA2, PARK7, HERC2, MYB, TCF4 and RNF168 (PubMed : 12223491, PubMed : 12511558, PubMed : 12631292, PubMed : 12727872, PubMed : 12750312, PubMed : 15831457, PubMed : 15976810, PubMed : 16617055, PubMed : 22508508, PubMed : 34048572). Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway (PubMed : 11388671). Involved in gene silencing (PubMed : 11248056). In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations (PubMed : 12727872, PubMed : 15831457). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed : 21965678). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs) (By similarity). Catalyzes conjugation of SUMO2 to KAT5 in response to DNA damage, facilitating repair of DNA double-strand breaks (DSBs) via homologous recombination (HR) (PubMed : 32832608). Mediates sumoylation of PARP1 in response to PARP1 trapping to chromatin (PubMed : 35013556). Mediates sumoylation of KLF8, repressiing KLF8 transcriptional activity and cell cycle progression into G(1) phase (PubMed : 16617055). Sumoylates ALKBH5 downstream of MAPK8/JNK1 and MAPK9/JNK2 in response to reactive oxygen species (ROS), inhibiting ALKBH5 RNA demethylase activity (PubMed : 34048572).
See full target information PIAS4
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:674 PubMed39277582

2024

Impaired SUMOylation of FoxA1 promotes nonalcoholic fatty liver disease through down-regulation of Sirt6.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Zou,Jinwen Liao,Min Xiao,Liang Liu,Dongling Dai,Mingguo Xu

Frontiers in pharmacology 13:872057 PubMed35559248

2022

Dimethyl Fumarate Ameliorates Doxorubicin-Induced Cardiotoxicity By Activating the Nrf2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoliang Hu,Cheng Li,Qian Wang,Zhixing Wei,Taizhong Chen,Yuepeng Wang,Yigang Li

Antioxidants & redox signaling 32:636-655 PubMed31903784

2020

Regulation of the 20S Proteasome by a Novel Family of Inhibitory Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Maya A Olshina,Galina Arkind,Fanindra Kumar Deshmukh,Irit Fainer,Mark Taranavsky,Daniel Hayat,Shifra Ben-Dor,Gili Ben-Nissan,Michal Sharon

Journal of molecular cell biology 11:356-370 PubMed29868881

2018

Muscle RING-finger protein-1 (MuRF1) functions and cellular localization are regulated by SUMO1 post-translational modification.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel Heras,Arvind Venkat Namuduri,Leonardo Traini,Ganna Shevchenko,Alexander Falk,Sara Bergström Lind,Mi Jia,Geng Tian,Stefano Gastaldello
View all publications
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