AB109596

Anti-E2F8抗体

Name: E2F8抗体 (ab109596)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109596
Price: 4845 CNY
Availability: InStock

Anti-E2F8 antibody

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(13 Publications)

Rabbit Polyclonal E2F8 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human Transcription factor E2F8 aa 800 to C-terminus.

查看别名

Transcription factor E2F8, E2F-8, E2F8

3 Images

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Immunoprecipitation - Anti-E2F8 antibody (AB109596)

Detection of E2F8 by Western Blot of Immunprecipitate.
ab109596 at 0.1 μg/mL staining E2F8 in Jurkat whole cell lysate immunoprecipitated using ab109596 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 3 seconds

All lanes:

Immunoprecipitation - Anti-E2F8 antibody (ab109596)

Predicted band size: 94 kDa

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Western blot - Anti-E2F8 antibody (AB109596)

All lanes:

Western blot - Anti-E2F8 antibody (ab109596) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 5 µg

Lane 4:

HeLa whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 94 kDa

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Exposure time: 3min

CiteAb

Western blot - Anti-E2F8 antibody (AB109596)

E2F8 western blot using anti-E2F8 antibody ab109596. Publication image and figure legend from Weijts, B. G., van Impel, A., et al., 2013, PLoS One, PubMed 24069224.

ab109596 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab109596 please see the product overview.

E2F7/8 directly regulate CCBE1 and FLT4 expression.A, Genes associated with the gene ontology (GO) term angiogenesis were extracted from the Sox2-cre;E2f7loxP/-E2f8loxP/- vs. wild-type E10.5 mouse embryos (p<0.05) database (GEO : GSE30488) and additionally analyzed for E2F binding sites and their presence in GO lymphangiogenesis (GO : 0001946). B, E2F binding elements within the CCBE1 and FLT4 promoter. Average and canonical E2F binding consensus. C, E2F7 and E2F8 ChIP performed on CCBE1 and FLT4 promoters in HeLa cells, arrows in (B) indicate primers used for ChIP. A previous reported E2F site within the E2F1 promoter and a non-specific site upstream (+700 bp) served as controls. D, mRNA and protein levels of E2f7-EGFP inducible HeLa cells treated with doxycyclin (0.2 μg /ml) for 24 hours. E, mRNA and protein levels of HeLa cells treated with scrambled or E2f7/8 siRNAs. Fold change in protein were calculated using TUBULIN as reference. Data presented as the average (±s.e.m.) compared to the control condition in three independent experiments.

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human Transcription factor E2F8 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

A0AVK6

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

E2F8 also known as transcription factor E2F8 plays a vital role in cell cycle regulation. This protein has a molecular weight of approximately 97 kDa. E2F8 is widely expressed in various tissues with significant levels detected in the liver and placenta. As part of the E2F family it functions as a transcription factor repressing or promoting the transcription of target genes involved in cell proliferation.

Biological function summary

E2F8 acts as a regulator within the context of the cell cycle. It forms a complex with other E2F family members such as E2F7 which can bind to specific DNA sequences and regulate the transcription of genes necessary for the G1/S transition. This activity makes E2F8 essential for controlling cell growth and maintaining cellular homeostasis. E2F8's ability to act as a repressor distinguishes it from other E2Fs which typically activate transcription.

Pathways

E2F8 integrates into the mechanistic framework of the cell cycle and DNA repair pathways. Its activity is closely tied to the p53 pathway where it can influence p53-mediated transcriptional repression. E2F8 influences other family proteins like E2F1 which are important in driving cell cycle progression and apoptosis. By modulating these pathways E2F8 fine-tunes the balance between cell division and cell death—an important aspect of normal development and tumor suppression.

E2F8 has a notable connection with cancer and developmental disorders. Its dysregulation has been observed in various cancers such as hepatocellular carcinoma where it can promote oncogenic transformation by altering cell cycle dynamics. E2F8 also interacts with proteins like CCNE1 which is often overexpressed in tumors. Furthermore abnormalities in E2F8 function can contribute to birth defects by disrupting normal cell cycle control during embryonic development.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Atypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1 : component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator : associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene.

See full target information Transcription factor E2F8

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Molecules and cells 47:100148 PubMed39537030

2024

E2F3a activates Gadd45b gene expression through PPARα-mediated transcriptional regulation in hepatic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Min Seok Woo,Atif Ali Khan Khalil,Frank J Gonzalez,Jung-Hwan Kim

Nature communications 13:6069 PubMed36241625

2022

Opposing functions of circadian protein DBP and atypical E2F family E2F8 in anti-tumor Th9 cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-A Park,Yun-Ji Lim,Wai Lim Ku,Dunfang Zhang,Kairong Cui,Liu-Ya Tang,Cheryl Chia,Peter Zanvit,Zuojia Chen,Wenwen Jin,Dandan Wang,Junji Xu,Ousheng Liu,Fu Wang,Alexander Cain,Nancy Guo,Hiroko Nakatsukasa,Chuan Wu,Ying E Zhang,Keji Zhao,WanJun Chen

Cell death & disease 13:230 PubMed35279688

2022

YTHDF1 promotes breast cancer cell growth, DNA damage repair and chemoresistance.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Sun,Dan Dong,Yuhong Xia,Liying Hao,Wei Wang,Chenghai Zhao

Journal of cellular physiology 236:4050-4065 PubMed33174204

2020

GBE attenuates arsenite-induced hepatotoxicity by regulating E2F1-autophagy-E2F7a pathway and restoring lysosomal activity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiong Chen,Dapeng Wang,Baofei Sun,Chunyan Liu,Kai Zhu,Aihua Zhang

Frontiers in oncology 10:561287 PubMed33224876

2020

Small Nuclear Ribonucleoprotein Polypeptide N Accelerates Malignant Progression and Poor Prognosis in Colorectal Cancer Transcriptionally Regulated by E2F8.

Applications

Unspecified application

Species

Unspecified reactive species

Meiling Ji,Li Ren,Yang Lv,Xinyuan Lao,Qingyang Feng,Wentao Tang,Aobo Zhuang,Tianyu Liu,Peng Zheng,Jianmin Xu

Scientific reports 10:4077 PubMed32139766

2020

Cryptochromes modulate E2F family transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Alanna B Chan,Anne-Laure Huber,Katja A Lamia

Journal of translational medicine 17:427 PubMed31878977

2019

The regulatory effect of microRNA-21a-3p on the promotion of telocyte angiogenesis mediated by PI3K (p110α)/AKT/mTOR in LPS induced mice ARDS.

Applications

Unspecified application

Species

Unspecified reactive species

Yile Zhou,Yajie Yang,Tao Liang,Yan Hu,Haihong Tang,Dongli Song,Hao Fang

Oncotarget 8:101509-101519 PubMed29254182

2017

Metformin induces cell cycle arrest at the G1 phase through E2F8 suppression in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Hao Jin,Yujin Kim,Bo Bin Lee,Joungho Han,Hong Kwan Kim,Young Mog Shim,Duk-Hwan Kim

The Journal of clinical investigation 126:2955-69 PubMed27454291

2016

E2f8 mediates tumor suppression in postnatal liver development.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsey N Kent,Jessica B Rakijas,Shusil K Pandit,Bart Westendorp,Hui-Zi Chen,Justin T Huntington,Xing Tang,Sooin Bae,Arunima Srivastava,Shantibhusan Senapati,Christopher Koivisto,Chelsea K Martin,Maria C Cuitino,Miguel Perez,Julian M Clouse,Veda Chokshi,Neelam Shinde,Raleigh Kladney,Daokun Sun,Antonio Perez-Castro,Ramadhan B Matondo,Sathidpak Nantasanti,Michal Mokry,Kun Huang,Raghu Machiraju,Soledad Fernandez,Thomas J Rosol,Vincenzo Coppola,Kamal S Pohar,James M Pipas,Carl R Schmidt,Alain de Bruin,Gustavo Leone

Human molecular genetics 25:2789-2800 PubMed27149985

2016

EZH2 is overexpressed in adrenocortical carcinoma and is associated with disease progression.

Applications

Unspecified application

Species

Unspecified reactive species

Coralie Drelon,Annabel Berthon,Mickael Mathieu,Bruno Ragazzon,Rork Kuick,Houda Tabbal,Amandine Septier,Stéphanie Rodriguez,Marie Batisse-Lignier,Isabelle Sahut-Barnola,Typhanie Dumontet,Jean-Christophe Pointud,Anne-Marie Lefrançois-Martinez,Silvère Baron,Thomas J Giordano,Jérôme Bertherat,Antoine Martinez,Pierre Val

View all publications

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Anti-E2F8抗体

Anti-E2F8 antibody

Immunoprecipitation - Anti-E2F8 antibody (AB109596)

Western blot - Anti-E2F8 antibody (AB109596)

Western blot - Anti-E2F8 antibody (AB109596)