AB11952

Anti-E2F6抗体[TFE61]

Anti-E2F6 antibody [TFE61]

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(7 Publications)

Mouse Monoclonal E2F6 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human E2F6.

查看别名

Transcription factor E2F6, E2F-6, E2F6

1 Images

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Western blot - Anti-E2F6 antibody [TFE61] (AB11952)

Detection of E2F6 by Western blot analysis. E2F6 is detected in nuclear extracts derived from K-562 cells using a 1/1000 dilution of ab11952.

Detection of E2F6 by Western blot analysis. E2F6 is detected in nuclear extracts

All lanes:

Western blot - Anti-E2F6 antibody [TFE61] (ab11952)

Predicted band size: 32 kDa

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关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

TFE61

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Recombinant Full Length Protein corresponding to Human E2F6.

O75461

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.02% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

E2F6 a member of the E2F family of transcription factors acts as a transcriptional repressor. It regulates genes essential for cell cycle progression particularly those involved in DNA replication. E2F6 lacks the transactivation domain present in other E2F family members allowing it uniquely to repress transcription without activating it. E2F6 has a molecular mass of approximately 30 kDa. The protein is mostly expressed in the nucleus of proliferating cells but it is also found in certain other tissues including the brain and testes.

Biological function summary

E2F6 plays an important role in regulating the cell cycle. It belongs to a multiprotein complex called the repressive E2F6 complex or PRC1 (Polycomb Repressive Complex 1). This complex is responsible for chromatin remodeling and transcriptional repression of target genes important in S phase entry and mitosis. By repressing specific E2F-responsive genes E2F6 inhibits excessive cell proliferation therefore maintaining proper cell cycle control.

Pathways

E2F6 contributes to important pathways like the cell cycle and DNA damage response pathways. In these pathways it is involved in controlling the transition phases of the cell cycle particularly regulating entry into the S phase. E2F6 interacts with proteins such as Rb (retinoblastoma protein) and other E2F members like E2F1 and E2F4 to control cell growth and ensure genome integrity.

Dysregulation of E2F6 has links to cancer and developmental disorders. In cancer overexpression or mutations affecting E2F6 can lead to uncontrolled cell proliferation due to improper repression of cell cycle genes. Additionally defects in the E2F6 gene have associations with developmental syndromes through interactions with other proteins like EZH2 a component of the Polycomb group proteins. These associations highlight the importance of E2F6 in both normal cell function and disease states.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

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靶点信息

Inhibitor of E2F-dependent transcription (PubMed : 9501179, PubMed : 9689056, PubMed : 9704927). Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' (PubMed : 9501179). Has a preference for the 5'-TTTCCCGC-3' E2F recognition site (PubMed : 9501179). E2F6 lacks the transcriptional activation and pocket protein binding domains (PubMed : 9501179, PubMed : 9704927). Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression (PubMed : 9501179, PubMed : 9689056). Represses expression of some meiosis-specific genes, including SLC25A31/ANT4 (By similarity). May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase (PubMed : 9501179).

See full target information E2F6

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

NPJ Regenerative medicine 7:14 PubMed35110584

2022

CDH18 is a fetal epicardial biomarker regulating differentiation towards vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Junghof,Yuta Kogure,Tian Yu,Eva María Verdugo-Sivianes,Megumi Narita,Antonio Lucena-Cacace,Yoshinori Yoshida

Oncology research 28:945-959 PubMed34588094

2021

Long Noncoding RNA LAMTOR5-AS1 Interference Affects MicroRNA-506-3p/E2F6-Mediated Behavior of Non-Small Cell Lung Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Guojie Chen,Kai Wang,Guoshu Li,Leidong Wang,Yangyang Xiao,Bo Chen

Annals of translational medicine 7:784 PubMed32042800

2020

LncRNA MATN1-AS1 prevents glioblastoma cell from proliferation and invasion via RELA regulation and MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Na Han,Li Yang,Xiaoxi Zhang,Yangmei Zhou,Rui Chen,Yang Yu,Zhen Dong,Mengxian Zhang

PLoS pathogens 12:e1005844 PubMed27548379

2016

EBV Nuclear Antigen 3C Mediates Regulation of E2F6 to Inhibit E2F1 Transcription and Promote Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Yonggang Pei,Shuvomoy Banerjee,Zhiguo Sun,Hem Chandra Jha,Abhik Saha,Erle S Robertson

Scientific reports 5:18016 PubMed26657798

2015

Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells.

Applications

Unspecified application

Species

Unspecified reactive species

Henning Gram Hansen,Claes Nymand Nilsson,Anne Mathilde Lund,Stefan Kol,Lise Marie Grav,Magnus Lundqvist,Johan Rockberg,Gyun Min Lee,Mikael Rørdam Andersen,Helene Faustrup Kildegaard

Carcinogenesis 31:766-76 PubMed19843643

2009

miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma.

Applications

Species

Human

Mayuko Furuta,Ken-ich Kozaki,Shinji Tanaka,Shigeki Arii,Issei Imoto,Johji Inazawa

Cancer research 68:2094-105 PubMed18381414

2008

Exploration of tumor-suppressive microRNAs silenced by DNA hypermethylation in oral cancer.

Applications

Species

Human

Ken-ichi Kozaki,Issei Imoto,Seiki Mogi,Ken Omura,Johji Inazawa

View all publications

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Anti-E2F6抗体[TFE61]

Anti-E2F6 antibody [TFE61]

Western blot - Anti-E2F6 antibody [TFE61] (AB11952)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原