Anti-E2F6抗体(ab53061)
Key features and details
- Rabbit polyclonal to E2F6
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-E2F6抗体
参阅全部 E2F6 一抗 -
描述
兔多克隆抗体to E2F6 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human E2F6 aa 141-190.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53061于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
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| IHC-P |
1/50 - 1/100.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
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IHC-P
1/50 - 1/100. |
靶标
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功能
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. -
组织特异性
Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen. -
序列相似性
Belongs to the E2F/DP family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1876 Human
- Entrez Gene: 50496 Mouse
- Omim: 602944 Human
- SwissProt: O75461 Human
- SwissProt: O54917 Mouse
- Unigene: 603093 Human
- Unigene: 23296 Mouse
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别名
- E2F 6 antibody
- E2F binding site modulating activity protein antibody
- E2F transcription factor 6 antibody
see all
图片
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Western blot - Anti-E2F6 antibody (ab53061)All lanes : Anti-E2F6 antibody (ab53061) at 1/500 dilution
Lane 1 : Extracts of HepG2 cells
Lane 2 : Extracts of HepG2 cells treated with peptide
Predicted band size: 31 kDa
Observed band size: 31 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F6 antibody (ab53061)Immunohistochemistry analysis of paraffin-embedded human brain tissue labeling E2F6 with ab53061. The picture on the right is blocked with the synthesized peptide. -
Western blot - Anti-E2F6 antibody (ab53061)Anti-E2F6 antibody (ab53061) at 1/500 dilution + NIH/3T3 cell lysate
Predicted band size: 31 kDa -
Western blot - Anti-E2F6 antibody (ab53061)Anti-E2F6 antibody (ab53061) at 1/500 dilution + HepG2 cell lysate
Predicted band size: 31 kDa -
Western blot - Anti-E2F6 antibody (ab53061)This image is courtesy of an Abreview submitted by Dr. Ligia TavaresAll lanes : Anti-E2F6 antibody (ab53061) at 1/500 dilution (in TBS fro 16 hours at 4°C )
Lane 1 : HeLa cells
Lane 2 : Cell lysate (nuclear) of mouse embryonic stem cells at 100000 cells
Secondary
All lanes : HRP-conjugated Donkey anti rabbit IgG polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 31 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
Blocking Step: 4% Milk for 1 hour at 22°C
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab53061 被引用在 11 文献中.
- Suzuki MM et al. TUG1-mediated R-loop resolution at microsatellite loci as a prerequisite for cancer cell proliferation. Nat Commun 14:4521 (2023). PubMed: 37607907
- Tao F et al. An optimized chromatin immunoprecipitation protocol using Staph-seq for analyzing genome-wide protein-DNA interactions. STAR Protoc 3:101918 (2022). PubMed: 36595937
- Dahlet T et al. E2F6 initiates stable epigenetic silencing of germline genes during embryonic development. Nat Commun 12:3582 (2021). PubMed: 34117224
- Mathsyaraja H et al. Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness. Elife 10:N/A (2021). PubMed: 34236315
- Liu L et al. The COX10-AS1/miR-641/E2F6 Feedback Loop Is Involved in the Progression of Glioma. Front Oncol 11:648152 (2021). PubMed: 34381702