重组Anti-E2F1抗体[S03-5G3] (ab317385)
![Western blot - Anti-E2F1 Antibody [S03-5G3] (ab317385)](https://www.abcam.cn/ps/products/317/ab317385/Images/ab317385-536482-anti-e2f1-antibody-s03-5g3-western-blot-c6-3t3-rat-mouse.jpg "Western blot - Anti-E2F1 Antibody [S03-5G3] (ab317385)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [S03-5G3] to E2F1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
概述
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产品名称
Anti-E2F1抗体[S03-5G3]
参阅全部 E2F1 一抗 -
描述
兔单克隆抗体[S03-5G3] to E2F1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human E2F1.
Database link: Q01094 -
阳性对照
- WB: C6 and NIH/3T3 cell lysate. ICC/IF: HeLa cells. IHC-P: Human tonsil tissue.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA -
Concentration information loading... -
纯度
Affinity purified -
克隆
单克隆 -
克隆编号
S03-5G3 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317385于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 47 kDa.
|
|
| IHC-P |
1/20.
|
|
| ICC/IF |
1/50.
|
| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 47 kDa. |
|
IHC-P
1/20. |
|
ICC/IF
1/50. |
靶标
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功能
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. -
序列相似性
Belongs to the E2F/DP family. -
翻译后修饰
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1869 Human
- Entrez Gene: 13555 Mouse
- Entrez Gene: 399489 Rat
- Omim: 189971 Human
- SwissProt: Q01094 Human
- SwissProt: Q61501 Mouse
- SwissProt: O09139 Rat
- Unigene: 654393 Human
see all -
别名
- Dmel\CG6376 antibody
- Dmel_CG6376 antibody
- drosE2F1 antibody
see all
图片
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Western blot - Anti-E2F1 Antibody [S03-5G3] (ab317385)All lanes : Anti-E2F1 Antibody [S03-5G3] (ab317385) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : NIH/3T3 cell lysate
Predicted band size: 47 kDaObserved band is higher than predicted band - this pattern is seen in other western blots for other antibodies to this target.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 Antibody [S03-5G3] (ab317385)](https://www.abcam.cn/ps/products/317/ab317385/Images/ab317385-536481-anti-e2f1-antibody-s03-5g3-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 Antibody [S03-5G3] (ab317385)Immunohistochemistry of E2F1 in paraffin-embedded human tonsil using ab317385 at dilution 1/2.
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![Immunocytochemistry/ Immunofluorescence - Anti-E2F1 Antibody [S03-5G3] (ab317385)](https://www.abcam.cn/ps/products/317/ab317385/Images/ab317385-536480-anti-e2f1-antibody-s03-5g3-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-E2F1 Antibody [S03-5G3] (ab317385)Immunofluorescence of E2F1 (green) in HeLa cells using ab317385 at dilution 1/50, and DAPI (blue).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317385 尚未被引用在任何文献中。