Anti-E2F1抗体[KH95] (ab4070)
![Western blot - Anti-E2F1 antibody [KH95] (ab4070)](https://www.abcam.cn/ps/products/4/ab4070/Images/ab4070-451727-anti-e2f1-antibody-kh95-western-blot-wildtype-hek293-e2f1-knockout-hek293-wildtype-hek293-e2f1-knockout-hek29.jpg "Western blot - Anti-E2F1 antibody [KH95] (ab4070)")
Key features and details
- Mouse monoclonal [KH95] to E2F1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
概述
-
产品名称
Anti-E2F1抗体[KH95]
参阅全部 E2F1 一抗 -
描述
小鼠单克隆抗体[KH95] to E2F1 -
宿主
Mouse -
特异性
Protein of 60 kDa. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human E2F1.
-
阳性对照
- WB: HepG2, HEK-293, HeLa and U-2 OS cell lysates. IHC-P: Human tonsil tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: Ascites, 1% BSA -
Concentration information loading... -
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
KH95 -
同种型
IgG2a -
研究领域
相关产品
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500. Predicted molecular weight: 47 kDa.
|
|
| IHC-P |
1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/500. Predicted molecular weight: 47 kDa. |
|
IHC-P
1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
-
功能
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. -
序列相似性
Belongs to the E2F/DP family. -
翻译后修饰
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 1869 Human
- Omim: 189971 Human
- SwissProt: Q01094 Human
- Unigene: 654393 Human
-
别名
- Dmel\CG6376 antibody
- Dmel_CG6376 antibody
- drosE2F1 antibody
see all
图片
-
Western blot - Anti-E2F1 antibody [KH95] (ab4070)All lanes : Anti-E2F1 antibody [KH95] (ab4070) at 1/500 dilution
Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : E2F1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Lanes 1 - 5: Merged signal (red and green). Green - ab4070 observed at 55 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.
ab4070 was shown to react with E2F1 in wild-type HEK-293 cells in Western blot with loss of signal observed in E2F1 knockout sample. Wild-type HEK-293 and E2F1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab4070 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody [KH95] (ab4070)](https://www.abcam.cn/ps/products/4/ab4070/Images/ab4070_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody [KH95] (ab4070)ab4070 staining human tonsil by IHC-P.
数据表及文件
-
Datasheet download
文献 (11)
ab4070 被引用在 11 文献中.
- Meng X et al. Dual functionalized brain-targeting nanoinhibitors restrain temozolomide-resistant glioma via attenuating EGFR and MET signaling pathways. Nat Commun 11:594 (2020). PubMed: 32001707
- Wang H et al. Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma. J Exp Clin Cancer Res 39:216 (2020). PubMed: 33054826
- Zou Y et al. ERK Inhibitor Enhances Everolimus Efficacy through the Attenuation of dNTP Pools in Renal Cell Carcinoma. Mol Ther Nucleic Acids 14:550-561 (2019). PubMed: 30771617
- Tian T et al. Long Noncoding RNA MPRL Promotes Mitochondrial Fission and Cisplatin Chemosensitivity via Disruption of Pre-miRNA Processing. Clin Cancer Res 25:3673-3688 (2019). PubMed: 30885939
- Bervoets S et al. Transcriptional dysregulation by a nucleus-localized aminoacyl-tRNA synthetase associated with Charcot-Marie-Tooth neuropathy. Nat Commun 10:5045 (2019). PubMed: 31695036
