Anti-E2 tag抗体[3F12] (ab978)
![Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [3F12] (ab978)](https://www.abcam.cn/ps/datasheet/Images/0/ab978/ab977_2.jpg "Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [3F12] (ab978)")
Key features and details
- Mouse monoclonal [3F12] to E2 tag
- Suitable for: WB, IP, ICC/IF
- Reacts with: Species independent
- Isotype: IgG1
概述
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产品名称
Anti-E2 tag抗体[3F12]
参阅全部 E2 tag 一抗 -
描述
小鼠单克隆抗体[3F12] to E2 tag -
宿主
Mouse -
经测试应用
适用于: WB, IP, ICC/IFmore details -
种属反应性
与反应: Species independent -
免疫原
Recombinant full length protein corresponding to Bovine papillomavirus E2 tag.
Database link: P03122 -
表位
GVSSTSSDFRDR(linear) -
常规说明
E2TAG having sequence SSTSSDFRDR or GVSSTSSDFRDR and antibodies specific for the peptide tag are covered by PCT Patent No PCT/EE01/00001 held by Quattromed Ltd. End-user is granted to use the licenced technology solely for internal research purpose only. This antibody should be used in conjunction with the E2 tagging vectors (links to the appropriate datasheet can be found in the Related Products section at the bottom of the datasheet).The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
3F12 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| IP | ||
| ICC/IF |
| 说明 |
|---|
IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
WB: 1/1000 - 1/5000 (1hr, RT) (dilute in 1% non-fat dry milk in TBST).
(Background can be reduced by adding NaCl up to a concentration of 1.5 M.)
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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相关性
The E2Tag is a peptide which is derived from the Bovine Pappillomavirus type-1 transactivator protein E2 and consists of 10 amino acids(SSTSSDFRDR). The detection of E2-tagged proteins is based on the binding of mouse monoclonal antibodies specific to the tagged recombinant protein. -
别名
- E2 epitope tag antibody
- SSTSSDFRDR epitope tag antibody
- SSTSSDFRDR tag antibody
图片
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Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [3F12] (ab978)
Staining of transfected COS-7 cells with COX-2 specific antibody (A, B, D) and with E2Tag-specific antibody (3F12)(C).
A - pcDNA3.1 rabbit COX-2,
B - pCG-E2Tag-COX,
C - pCG-COX[E2Tag]
D – neg. control, carrier DNA alone
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab978 被引用在 7 文献中.
- Wu K et al. Cell fate determination factor DACH1 inhibits c-Jun-induced contact-independent growth. Mol Biol Cell 18:755-67 (2007). PubMed: 17182846
- Auvinen E et al. Human papillomavirus type 16 E5 protein colocalizes with the antiapoptotic Bcl-2 protein. Arch Virol 149:1745-59 (2004). PubMed: 15593417
- Ord D & Ord T Mouse NIPK interacts with ATF4 and affects its transcriptional activity. Exp Cell Res 286:308-20 (2003). PubMed: 12749859
- Koljak R et al. The basis of prostaglandin synthesis in coral: molecular cloning and expression of a cyclooxygenase from the Arctic soft coral Gersemia fruticosa. J Biol Chem 276:7033-40 (2001). PubMed: 11085996
- Kaldalu N et al. Functional domains of the TOL plasmid transcription factor XylS. J Bacteriol 182:1118-26 (2000). PubMed: 10648539
- Kaldalu N et al. Monitoring and purification of proteins using bovine papillomavirus E2 epitope tags. Biotechniques 28:456-60, 462 (2000). PubMed: 10723557
- Kurg R et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). PubMed: 10233926