重组Anti-E1 Ubiquitin Activating Enzyme 1/UBA1抗体[EPR14203(B)] (ab180125)
![Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)](https://www.abcam.cn/ps/products/180/ab180125/Images/ab180125-484472-AntiE1-Ubiquitin-Activating-Enzyme-1UBA1-antibody-EPR14203-B-Western-blot-Recombinant-human-protein.png "Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14203(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1抗体[EPR14203(B)]
参阅全部 E1 Ubiquitin Activating Enzyme 1/UBA1 一抗 -
描述
兔单克隆抗体[EPR14203(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human E1 Ubiquitin Activating Enzyme 1/UBA1 aa 200-300. The exact sequence is proprietary.
Database link: P22314 -
阳性对照
- Fetal brain, K562, HeLa and Jurkat cell lysates; Human ovarian carcinoma tissue; Jurkat and K562 cells, GST-tagged full-length recombinant human UBA1 and UBA7 proteins.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14203(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180125于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/70.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 118 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/100.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 118 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/100. |
靶标
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功能
Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Defects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2) [MIM:301830]; also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures. -
序列相似性
Belongs to the ubiquitin-activating E1 family. -
翻译后修饰
ISGylated. - Information by UniProt
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数据库链接
- Entrez Gene: 7317 Human
- Entrez Gene: 22201 Mouse
- Entrez Gene: 314432 Rat
- Omim: 314370 Human
- SwissProt: P22314 Human
- SwissProt: Q02053 Mouse
- SwissProt: Q5U300 Rat
- Unigene: 533273 Human
see all -
别名
- A1S9 antibody
- A1S9 protein antibody
- A1S9T and BN75 temperature sensitivity complementing antibody
see all
图片
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Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125) at 1/1000 dilution
Lane 1 : GST tagged Recombinant Human UBA7(Ube1L) protein (Full length, 166 KDa)
Lane 2 : GST tagged Recombinant Human UBA1 protein (Full length, 146 KDa)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 118 kDa
Observed band size: 146,166 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and Diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)](https://www.abcam.cn/ps/products/180/ab180125/Images/ab180125-210240-anti-e1-ubiquitin-activating-enzyme-antibody-epr14203b-western-blot.jpg)
Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125) at 1/5000 dilution
Lane 1 : Fetal brain tissue lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 118 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)](https://www.abcam.cn/ps/products/180/ab180125/Images/ab180125-210243-anti-e1-ubiquitin-activating-enzyme-antibody-epr14203b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedK562 cells labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/70 dilution (red) compared toa rabbit IgGcontrol (green), followed by Goat anti rabbit IgG (FITC) secondary antibodyat 1/75 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)](https://www.abcam.cn/ps/products/180/ab180125/Images/ab180125-210242-anti-e1-ubiquitin-activating-enzyme-antibody-epr14203b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)Immunofluorescent analysis of acetone-fixed Jurkat cells labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/100 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution. Counter stained with DAPI (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)](https://www.abcam.cn/ps/products/180/ab180125/Images/ab180125-210241-anti-e1-ubiquitin-activating-enzyme-antibody-epr14203b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)](https://www.abcam.cn/ps/products/180/ab180125/Images/ab180125-5-benefits-of-recombinant-antibodies.png)
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab180125 被引用在 2 文献中.
- Chen Z et al. CircGPRC5A enhances colorectal cancer progress by stabilizing PPP1CA and inducing YAP dephosphorylation. J Exp Clin Cancer Res 42:334 (2023). PubMed: 38057879
- Wei Z et al. Comparative proteomic analysis identifies differentially expressed proteins and reveals potential mechanisms of traumatic heterotopic ossification progression. J Orthop Translat 34:42-59 (2022). PubMed: 35615641