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Tags & Cell Markers Epitope Tags Other Tags E tag

Anti-E tag抗体(ab3397)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (8)

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Immunocytochemistry - Anti-E tag antibody (ab3397)

    Key features and details

    • Rabbit polyclonal to E tag
    • Suitable for: ICC
    • Reacts with: Species independent
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-E tag抗体
      参阅全部 E tag 一抗
    • 描述

      兔多克隆抗体to E tag
    • 宿主

      Rabbit
    • 经测试应用

      适用于: ICCmore details
    • 种属反应性

      与反应: Species independent
    • 免疫原

      Synthetic peptide corresponding to E tag conjugated to keyhole limpet haemocyanin.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.20
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Tags & Cell Markers
      • Epitope Tags
      • Other Tags
      • E tag

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3397于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC (1)
    Use at an assay dependent concentration.
    说明
    ICC
    Use at an assay dependent concentration.

    靶标

    • 相关性

      E tag is a peptide epitope tag, sequence GAPVPYPDPLEPR.
    • 别名

      • E epitope tag antibody
      • GAPVPYPDPLEPR epitope tag antibody
      • GAPVPYPDPLEPR tag antibody

    图片

    • Immunocytochemistry - Anti-E tag antibody (ab3397)
      Immunocytochemistry - Anti-E tag antibody (ab3397)This image is a courtesy of Anonymous Abreview

      ab3397 staining E tag in cultured human HUVEC cells by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton x100 and blocking with 1% BSA was done for 45 minutes at 250C. Samples were incubated with primary antibody (1/100: in 1% BSA/PBS) for 16 hours at 4°C. An FITC-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.

      See Abreview

    实验方案

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (8)

    发表研究结果有使用 ab3397?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3397 被引用在 8 文献中.

    • McNutt PM  et al. Neuronal delivery of antibodies has therapeutic effects in animal models of botulism. Sci Transl Med 13:N/A (2021). PubMed: 33408188
    • Chekli Y  et al. Visualizing the dynamics of exported bacterial proteins with the chemogenetic fluorescent reporter FAST. Sci Rep 10:15791 (2020). PubMed: 32978420
    • Zhao L  et al. Intrabody against prolyl hydroxylase 2 promotes angiogenesis by stabilizing hypoxia-inducible factor-1a. Sci Rep 9:11861 (2019). PubMed: 31413262
    • Mont S  et al. Accumulation of isolevuglandin-modified protein in normal and fibrotic lung. Sci Rep 6:24919 (2016). IF . PubMed: 27118599
    • Chavanayarn C  et al. Humanized-single domain antibodies (VH/VHH) that bound specifically to Naja kaouthia phospholipase A2 and neutralized the enzymatic activity. Toxins (Basel) 4:554-67 (2012). WB . PubMed: 22852068
    • Leotoing L  et al. A20-binding inhibitor of nuclear factor-kappaB (NF-kappaB)-2 (ABIN-2) is an activator of inhibitor of NF-kappaB (IkappaB) kinase alpha (IKKalpha)-mediated NF-kappaB transcriptional activity. J Biol Chem 286:32277-88 (2011). IP . PubMed: 21784860
    • Chen TT  et al. Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. J Cell Biol 188:595-609 (2010). PubMed: 20176926
    • Lampugnani MG  et al. Vascular endothelial cadherin controls VEGFR-2 internalization and signaling from intracellular compartments. J Cell Biol 174:593-604 (2006). PubMed: 16893970

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Flow Cytometry abreview for Anti-E tag antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Saccharomyces cerevisiae Cell (live yeast cells)
    Specification
    live yeast cells
    Preparation
    Cell harvesting/tissue preparation method: harvest cells then wash with 1XPBS
    Sample buffer: 1XPBS
    Fixation
    No fixation
    Permeabilization
    No
    Gating Strategy
    E-tag expression
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 10 2012

    Immunocytochemistry abreview for Anti-E tag antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (HUVEC)
    Specification
    HUVEC
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% triton-X100
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 13 2009

    Question

    Has this product been tested for use in IHC-P?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 31 2005

    Answer

    Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards.

    Read More

    Abcam Scientific Support

    回复于 Mar 31 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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