Anti-E-Syt1抗体(ab118805)
Key features and details
- Rabbit polyclonal to E-Syt1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-E-Syt1抗体
参阅全部 E-Syt1 一抗 -
描述
兔多克隆抗体to E-Syt1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human E-Syt1 aa 1054-1104.
Database link: NP_056107.1 -
阳性对照
- HeLa, 293T and Jurkat whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab118805 was affinity purified using an epitope specific to E-Syt1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118805于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 123 kDa.
|
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| IP |
Use at 2-10 µg/mg of lysate.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 123 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
May play a role as calcium-regulated intrinsic membrane protein. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the extended synaptotagmin family.
Contains 5 C2 domains. -
细胞定位
Membrane. Localizes to intracellular membranes. - Information by UniProt
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数据库链接
- Entrez Gene: 23344 Human
- SwissProt: Q9BSJ8 Human
- Unigene: 632729 Human
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别名
- E Syt1 antibody
- E-Syt1 antibody
- Esyt1 antibody
see all
图片
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Western blot - Anti-E-Syt1 antibody (ab118805)All lanes : Anti-E-Syt1 antibody (ab118805) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 123 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-E-Syt1 antibody (ab118805)Detection of E-Syt1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118805 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 3 minutes.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab118805 被引用在 1 文献中.
- Li L et al. Sunitinib treatment promotes metastasis of drug-resistant renal cell carcinoma via TFE3 signaling pathway. Cell Death Dis 12:220 (2021). PubMed: 33637706