AB118805

Anti-E-Syt1抗体

Name: E-Syt1抗体 (ab118805)| Abcam中文官网
Brand: Abcam Limited
SKU: AB118805
Price: 4744 CNY
Availability: InStock

Anti-E-Syt1 antibody

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(3 Publications)

Rabbit Polyclonal E-Syt1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ESYT1 aa 1050 to C-terminus.

查看别名

FAM62A, KIAA0747, MBC2, ESYT1, Extended synaptotagmin-1, E-Syt1, Membrane-bound C2 domain-containing protein

2 Images

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Immunoprecipitation - Anti-E-Syt1 antibody (AB118805)

Detection of E-Syt1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118805 at 6 μg/mg lysate for IP (Lane 1) and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-E-Syt1 antibody (ab118805)

Predicted band size: 123 kDa

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Unknown

Western blot - Anti-E-Syt1 antibody (AB118805)

All lanes:

Western blot - Anti-E-Syt1 antibody (ab118805) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 123 kDa

true

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human ESYT1 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab118805 was affinity purified using an epitope specific to E-Syt1 immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

E-Syt1 also known as Extended Synaptotagmin 1 is a protein encoding a molecule of approximately 129 kDa. This protein plays an essential role in the tethering of the endoplasmic reticulum (ER) to the plasma membrane facilitating lipid transfer between these organelles. It is mostly expressed in the brain and other tissues. E-Syt1 possesses N-terminal transmembrane segments that anchor it within the ER membrane and its C-terminal part reaches out to bind PI(45)P2 at the plasma membrane upon increased cytosolic calcium levels.

Biological function summary

The Extended Synaptotagmin family which includes E-Syt1 forms a complex known as the E-Syt1/SMP complex which aids in the transfer of lipids for membrane repair and cellular homeostasis. The protein ensures cell membrane integrity by shuttling lipids in response to cellular signals. This mechanism supports cell survival and function especially under stress conditions where membrane damage may occur.

Pathways

E-Syt1 is integral to phosphoinositide and calcium signaling processes. It forms important interactions within the phosphoinositide metabolism pathway facilitating membrane dynamics. The protein also intersects with pathways involving calcium signaling due to its calcium-binding capacity working alongside proteins like Synaptotagmin to fine-tune cellular responses to fluctuations in calcium levels.

Extended Synaptotagmin 1 has implications in conditions like neurodegenerative diseases and certain cancers. Alterations in E-Syt1 activity or expression levels can contribute to conditions such as Alzheimer’s disease where disrupted lipid transport affects neuronal health. E-Syt1 in tandem with proteins like tau in Alzheimer’s highlights its role in maintaining cellular functions that prevent disease progression. Its involvement in lipid transfer can also relate to cancer pathologies where cell membrane dynamics are important.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport (By similarity). Binds calcium (via the C2 domains) and translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels. Helps tether the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane.

See full target information ESYT1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2135 PubMed40032835

2025

Proximity proteomics reveals a mechanism of fatty acid transfer at lipid droplet-mitochondria- endoplasmic reticulum contact sites.

Applications

Unspecified application

Species

Unspecified reactive species

Ayenachew Bezawork-Geleta,Camille J Devereux,Stacey N Keenan,Jieqiong Lou,Ellie Cho,Shuai Nie,David P De Souza,Vinod K Narayana,Nicole A Siddall,Carlos H M Rodrigues,Stephanie Portelli,Tenghao Zheng,Hieu T Nim,Mirana Ramialison,Gary R Hime,Garron T Dodd,Elizabeth Hinde,David B Ascher,David A Stroud,Matthew J Watt

Cell death & disease 12:220 PubMed33637706

2021

Sunitinib treatment promotes metastasis of drug-resistant renal cell carcinoma via TFE3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Luchao Li,Shuo Zhao,Zhengfang Liu,Nianzhao Zhang,Shuo Pang,Jikai Liu,Cheng Liu,Yidong Fan

EMBO molecular medicine 6:1593-609 PubMed25319547

2014

5-azacytidine inhibits nonsense-mediated decay in a MYC-dependent fashion.

Applications

Unspecified application

Species

Unspecified reactive species

Madhuri Bhuvanagiri,Joe Lewis,Kerstin Putzker,Jonas P Becker,Stefan Leicht,Jeroen Krijgsveld,Richa Batra,Brad Turnwald,Bogdan Jovanovic,Christian Hauer,Jana Sieber,Matthias W Hentze,Andreas E Kulozik

View all publications

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Anti-E-Syt1抗体

Anti-E-Syt1 antibody

Immunoprecipitation - Anti-E-Syt1 antibody (AB118805)

Western blot - Anti-E-Syt1 antibody (AB118805)