Anti-E. coli O + E. coli K抗体(ab31499)

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The immunogen was a mixture of intact and lysed bacteria which had not been denatured in any way. This antibody is reactive with all O and K antigenic serotypes of E. coli, therefore it will recognize the K12 strain. This antibody is purified by ammonium sulphate precipitation.

Thank you for contacting us. I have been in touch with the lab regarding your inquiry. I do wish to apologize for the delay in getting this information for you. Concerning ab31499, Anti-E. coli O + E. coli K antibody: The immunogen for this antibody is a mixture of the following E. coli strains: O157:H7, O20, O125, O55, O111 & K12. The mixture of these strains was of intact plus lysed bacteria which had not been denatured in any way. All E. coli have many surface and internal antigens in common and this antibody recognizes this large mixture and therefore reacts with most, if not all, E. coli. These antibodies have been tested against the strains listed above. These antibodies have not cross-absorbed and may react with related Enterobacteriaceae. Potential cross-reacting strains have not been identified. Because this antibody is directed against the surface components of E. coli, and Lipid A is one of these components, this my be the crossreacting target. Lipid A is broadly conserved in gram-negative bacteria, so both E. coli and Pseudomonas may be recognized. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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I can now share some more information relating to the antibodies we have directed towards E. coli (O and K). We have two different antibodies directed against this bacterium, with different conjugation available:

ab31499, unlabelled(ab33604, FITC; ab33837, HRP; ab33839, Biotin conjugated of same antibody preparation as ab31499)

The immunogen for these antibodies is a mixture of the following E. coli strains: O157:H7, O20, O125, O55, O111 & K12. The mixture of these strains was of intact plus lysed bacteria which had not been denatured in any way. All E. coli have many surface and internal antigens in common and this antibody recognizes this large mixture and therefore reacts with most, if not all, E. coli. Theseantibodies have been tested against the strains listed above but have not been cross-absorbed and may react with related Enterobacteriaceae. These potential cross-reacting strains have not been identified.

ab20765, ab20856 and ab21110 are Biotin, FITC and HRP conjugates of the same antibody.

These antibodies have been raised against a mixture of E. coli serotypes.These antibodies have been tested for reactivity against the immunogen strains of E.coli but again they have not been absorbed and will most likely cross react withother related Enterobacteriaceae.



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