重组Anti-E Cadherin抗体[EPR16845-108] (ab181296)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16845-108] to E Cadherin
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-E Cadherin抗体[EPR16845-108]
参阅全部 E Cadherin 一抗 -
描述
兔单克隆抗体[EPR16845-108] to E Cadherin -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: His-tagged mouse E-Cadherin active protein (aa1-709); Rat spleen lysate; Mouse plasma and serum; Mouse brain lysate. IP: Mouse serum.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR16845-108 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181296于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 120, 84 kDa (predicted molecular weight: 97 kDa).
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IP |
1/30.
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说明 |
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WB
1/1000. Detects a band of approximately 120, 84 kDa (predicted molecular weight: 97 kDa). |
IP
1/30. |
靶标
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功能
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. -
组织特异性
Non-neural epithelial tissues. -
疾病相关
Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease. -
序列相似性
Contains 5 cadherin domains. -
翻译后修饰
During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. -
细胞定位
Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 12550 Mouse
- Entrez Gene: 83502 Rat
- SwissProt: P09803 Mouse
- SwissProt: Q9R0T4 Rat
- Unigene: 35605 Mouse
- Unigene: 1303 Rat
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别名
- Arc 1 antibody
- CADH1_HUMAN antibody
- Cadherin 1 antibody
see all
图片
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Lane 1 : Anti-E Cadherin antibody [EPR16845-108] (ab181296) at 1/20000 dilution
Lane 2 : Anti-E Cadherin antibody [EPR16845-108] (ab181296) at 1/100000 dilution
All lanes : His-tagged mouse E-Cadherin active protein (aa1-709)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 97 kDa
Observed band size: 120,84 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
All lanes : Anti-E Cadherin antibody [EPR16845-108] (ab181296) at 1/1000 dilution
Lane 1 : Rat spleen lysate
Lane 2 : Mouse plasma
Lane 3 : Mouse serum
Lane 4 : Mouse brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1 & 4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 2-3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 97 kDa
Observed band size: 120,84 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1-2: 30 seconds; Lanes 3-4: 15 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 11076937; PMID: 11953314).
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E Cadherin was immunoprecipitated from 1 mg of mouse serum with ab181296 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181296 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse serum 10 µg (Input).
Lane 2: ab181296 IP in mouse serum.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab181296 in mouse serum.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (6)
ab181296 被引用在 6 文献中.
- Luo D et al. Pirfenidone suppressed triple-negative breast cancer metastasis by inhibiting the activity of the TGF-β/SMAD pathway. J Cell Mol Med 27:456-469 (2023). PubMed: 36651490
- Ye J et al. MicroRNA‑671‑5p inhibits cell proliferation, migration and invasion in non‑small cell lung cancer by targeting MFAP3L. Mol Med Rep 25:N/A (2022). PubMed: 34841435
- Jia M et al. Inhibition of PI3K/AKT/mTOR Signalling Pathway Activates Autophagy and Suppresses Peritoneal Fibrosis in the Process of Peritoneal Dialysis. Front Physiol 13:778479 (2022). PubMed: 35309056
- Tan Q et al. VEGF-B targeting by aryl hydrocarbon receptor mediates the migration and invasion of choriocarcinoma stem-like cells. Cancer Cell Int 22:221 (2022). PubMed: 35773697
- Peng J et al. Sema4C modulates the migration of primary tumor-associated lymphatic endothelial cells via an ERK-mediated pathway. Exp Ther Med 22:1102 (2021). PubMed: 34504556
- Zhu Z et al. Gut microbiota regulate tumor metastasis via circRNA/miRNA networks. Gut Microbes 12:1788891 (2020). PubMed: 32686598