Anti-Dystrophin抗体[MANDRA1] (ab7164)

Thank you for your inquiry.


Based on its primary structure, the dystrophin can be divided into four distinct structural domains: (1) the N-terminal actin-binding domain (aa 14-240), (2) the large triple helical spectrin-like domain (aa 253-3040) composed of 24 repeating units similar to those in β-spectrin, which are predicted to form triple-helical coiled-coils, (3) the cysteine-rich domain (aa 3080- 3360) and the C-terminal domain (aa 3361- 3685). http://www.sciencedirect.com/science/article/pii/0092867488903832


Please find information on the location of the immunogens by antibody below:


ab15278: C-terminal

ab154168: C-terminal

ab129996: epitope not mapped to our knowledge

ab14452: epitope not mapped to our knowledge

ab85302: spectrin-like domain/ rod

ab7164: C-terminal

ab15277: C-terminal


Please note the above antibodies are tested and guaranteed for different species and applications. Please choose carefully. If you let me know what species your samples are from and what application you are planning I will be happy to help choose the most suitable antibody.


Importantly, I also have to confirm that all our products are for research purposes only and are not intended for diagnostic or therapeutic use.

Thank you for your phone enquiries and interest in our products.

ab7163 - Anti-Dystrophin antibody [MANDYS8]

This antibody Monoclonal Anti-Dystrophin, clone MANDYS8, recognizes an epitope located on the rod domain of the human dystrophin molecule, The publications that are cited in the data sheet, which mapped the epitope that the MANDYS8 clone recognises are:

1. Nguyen, thi Man, et al., FEBS Lett., 262, 237 (1990).
2. Sedgwick, S., et al. Rapid mapping by transposon mutagenesis of epitopes on the muscular dystrophy protein, dystrophin Nucl. Acids Res. (1991) 19(21): 5889-5894


According to these references the epitope recognised by this clone lies between residues 1400 and 1505.

Regarding the epitopes of the other 3 antibodies, ab3149, ab7164, ab15277 - no information is available regarding yet. As soon as we have further details, we will update the corresponding datasheets.

I hope that this helps and please do not hesitate to contact us if you require any further information or assistance.

Thank you for your phone call. As we discussed, this antibody is not conjugated so it should not produce any signal in the absence of a secondary antibody. It is likely that the signal you are observing is autofluorescence. The attached reference details several common types of autofluorescence and tips for avoiding them. I hope this helps, please let me know if you have any additional questions.

Thank you for your email. It was nice to speak to you over the phone.
I am sorry to hear that you are encountering problems with ab7164.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1017104 with ab3149.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for your e-mail. I would recommend using the same fixative that have been used in the references where staining was done on frozen section; unfortunately I cannot access those papers myself to find this information. I would suspect the fixation will be ice cold acetone or methanol (or a mixture) for 10min, so something similar would be a good starting point. If you have enough cells I would recommend trying in parallel a well with 4%PFA fixing (for 10min) just in case this works in ICC. I hope this will help you, could you please let me know how you get on and do not hesitate to contact me if you need further advice on your staining protocol,

Thank you for your enquiry. The antibody will work in frozen sections and I would recommend the following references as sources of staining protocols as they have tested the antibody in IHC: Ellis JM et al. Specificity of dystrophin analysis improved with monoclonal antibodies. Lancet 336:881-2 (1990). PubMed: 1699095. Nguyen TM et al. Monoclonal antibodies for dystrophin analysis. Epitope mapping and improved binding to SDS-treated muscle sections. Biochem J 288 (Pt 2):663-8 (1992). PubMed: 1281410 Gussoni E et al. Normal dystrophin transcripts detected in Duchenne muscular dystrophy patients after myoblast transplantation. Nature 356:435-8 (1992). PubMed: 1557125 I hope these references will help, please let me know if you need further information,

Thank you for your phone call. This clone was tested for application in both direct and indirect ELISA as per the citations below (the PubMed links can be found on the online datasheet for ab7164). Morris GE et al. A quantitative ELISA for dystrophin. J Immunol Methods 161:23-8 (1993). PubMed: 8486926 Nguyen TM et al. Monoclonal antibodies for dystrophin analysis. Epitope mapping and improved binding to SDS-treated muscle sections. Biochem J 288 (Pt 2):663-8 (1992). PubMed: 1281410 These references may offer additional information pertaining to appropriate controls and methodology. If you have any additional questions, please contact us again.