Anti-Dyrk1B/MIRK抗体(ab113968)
Key features and details
- Rabbit polyclonal to Dyrk1B/MIRK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Dyrk1B/MIRK抗体 -
描述
兔多克隆抗体to Dyrk1B/MIRK -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
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免疫原
Synthetic peptide corresponding to Human Dyrk1B/MIRK aa 500-550.
Database link: NP_004705.1 -
阳性对照
- HeLa whole cell lysate (ab150035)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113968于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
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| IP |
Use at 5-15 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 69 kDa. |
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IP
Use at 5-15 µg/mg of lysate. |
靶标
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功能
Dual-specificity kinase which possesses both serine/ threonine and tyrosine kinase activities. Enhances the transcriptional activity of TCF1/HNF1A and FOXO1. Inhibits epithelial cell migration. Mediates colon carcinoma cell survival in mitogen-poor environments. -
组织特异性
Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated on tyrosine residues. Phosphorylated by MAP kinase. Tyrosine phosphorylation may be required for dimerization. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 507571 Cow
- Entrez Gene: 9149 Human
- Entrez Gene: 13549 Mouse
- Entrez Gene: 308468 Rat
- Entrez Gene: 698848 Rhesus monkey
- Omim: 604556 Human
- SwissProt: Q9Y463 Human
- SwissProt: Q9Z188 Mouse
see all -
别名
- AOMS3 antibody
- Dual specificity tyrosine (Y) phosphorylation regulated kinase 1B antibody
- Dual specificity tyrosine phosphorylation regulated kinase 1B antibody
see all
图片
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Western blot - Anti-Dyrk1B/MIRK antibody (ab113968)All lanes : Anti-Dyrk1B/MIRK antibody (ab113968) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 1 minute -
Immunoprecipitation - Anti-Dyrk1B/MIRK antibody (ab113968)Detection of Dyrk1B/MIRK in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab113968 at 10 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab113968 尚未被引用在任何文献中。