Anti-Dynamin 2抗体(ab3457)
Key features and details
- Rabbit polyclonal to Dynamin 2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human, Non human primates
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Dynamin 2抗体
参阅全部 Dynamin 2 一抗 -
描述
兔多克隆抗体to Dynamin 2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human, Non human primates
预测可用于: Cow
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免疫原
Synthetic peptide corresponding to Human Dynamin 2 aa 760-779.
Sequence:SPTPQRRPVSSIHPPGRPPA
(Peptide available asab4985) -
阳性对照
- WB: SH-SY5Y, PC-12, Neuro 2a, SK-N-AS, U-87 MG, rat brain tissue; ICC/IF: SH-SY5Y, HeLa; IHC: human lung tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
|
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| ICC/IF | (4) |
Use at an assay dependent concentration.
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| IHC-P |
Use a concentration of 1 µg/ml.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 1 µg/ml. |
靶标
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功能
Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis. -
组织特异性
Ubiquitously expressed. -
疾病相关
Defects in DNM2 are a cause of centronuclear myopathy autosomal dominant (ADCNM) [MIM:160150]; also known as autosomal dominant myotubular myopathy. Centronuclear myopathies (CNMs) are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. CNMs comprise a wide spectrum of phenotypes, ranging from severe neonatal to mild late-onset familial forms. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) [MIM:606482]. Charcot-Marie-Tooth disease (CMT) is a clinically and genetically heterogeneous disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. CMTDIB is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. -
序列相似性
Belongs to the dynamin family.
Contains 1 GED domain.
Contains 1 PH domain. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Microtubule-associated. Also found in the postsynaptic density of neuronal cells. - Information by UniProt
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数据库链接
- Entrez Gene: 1785 Human
- Entrez Gene: 13430 Mouse
- Entrez Gene: 25751 Rat
- Omim: 602378 Human
- SwissProt: P50570 Human
- SwissProt: P39054 Mouse
- SwissProt: P39052 Rat
- Unigene: 211463 Human
see all -
别名
- CMT2M antibody
- CMTDI1 antibody
- CMTDIB antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (ab3457)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y cells labeling Dynamin 2 with ab3457 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/2000 dilution (green). The nuclear counterstain is DAPI (blue), and F-actin is stained with Rhodamine Phalloidin iat 1/300 dilution (red). Negative control was cells without primary antibody present.
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Western blot - Anti-Dynamin 2 antibody (ab3457)All lanes : Anti-Dynamin 2 antibody (ab3457)
Lane 1 : SH-SY5Y whole cell lysate
Lane 2 : PC-12
Lane 3 : Neuro-2a
Lane 4 : SK-N-AS
Lane 5 : U- 87 MG
Lane 6 : Rat Brain
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugated -
Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (ab3457)Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HeLa cells labeling Dynamin 2 with ab3457 at 2 ug/ml followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/2000 dilution (green).
The nuclear counterstain is DAPI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 2 antibody (ab3457)ab3457 (1µg/ml) staining Dynamin 2 in human lung using an automated system (DAKO Autostainer Plus).
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (ab3457)ICC/IF image of ab3457 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3457, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (45)
ab3457 被引用在 45 文献中.
- Trochet D et al. Benefits of therapy by dynamin-2-mutant-specific silencing are maintained with time in a mouse model of dominant centronuclear myopathy. Mol Ther Nucleic Acids 27:1179-1190 (2022). PubMed: 35282416
- Dudhal S et al. Development of versatile allele-specific siRNAs able to silence all the dominant dynamin 2 mutations. Mol Ther Nucleic Acids 29:733-748 (2022). PubMed: 36090755
- Støle TP et al. The female syndecan-4-/- heart has smaller cardiomyocytes, augmented insulin/pSer473-Akt/pSer9-GSK-3β signaling, and lowered SCOP, pThr308-Akt/Akt and GLUT4 levels. Front Cell Dev Biol 10:908126 (2022). PubMed: 36092718
- Lammertse HCA et al. Reduced dynamin-1 levels in neurons lacking MUNC18-1. J Cell Sci 135:N/A (2022). PubMed: 36245272
- Ghosh M et al. CD13 is a critical regulator of cell-cell fusion in osteoclastogenesis. Sci Rep 11:10736 (2021). PubMed: 34031489