为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-Dynamin 2抗体(ab3457)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (40)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (ab3457)
  • Western blot - Anti-Dynamin 2 antibody (ab3457)
  • Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (ab3457)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 2 antibody (ab3457)
  • Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (ab3457)

Key features and details

  • Rabbit polyclonal to Dynamin 2
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human, Non human primates
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] (ab151555)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Dynamin 2抗体
    参阅全部 Dynamin 2 一抗
  • 描述

    兔多克隆抗体to Dynamin 2
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Human, Non human primates
    预测可用于: Cow
  • 免疫原

    Synthetic peptide corresponding to Human Dynamin 2 aa 760-779.
    Sequence:

    SPTPQRRPVSSIHPPGRPPA


    (Peptide available as ab4985)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: SH-SY5Y, PC-12, Neuro 2a, SK-N-AS, U-87 MG, rat brain tissue; ICC/IF: SH-SY5Y, HeLa; IHC: human lung tissue.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Dynamin 2 protein (ab132947)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3457于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration.
ICC/IF (4)
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 µg/ml.
说明
WB
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 µg/ml.

靶标

  • 功能

    Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.
  • 组织特异性

    Ubiquitously expressed.
  • 疾病相关

    Defects in DNM2 are a cause of centronuclear myopathy autosomal dominant (ADCNM) [MIM:160150]; also known as autosomal dominant myotubular myopathy. Centronuclear myopathies (CNMs) are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. CNMs comprise a wide spectrum of phenotypes, ranging from severe neonatal to mild late-onset familial forms. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
    Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) [MIM:606482]. Charcot-Marie-Tooth disease (CMT) is a clinically and genetically heterogeneous disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. CMTDIB is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • 序列相似性

    Belongs to the dynamin family.
    Contains 1 GED domain.
    Contains 1 PH domain.
  • 细胞定位

    Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Microtubule-associated. Also found in the postsynaptic density of neuronal cells.
  • Target information above from: UniProt accession P50570 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1785 Human
    • Entrez Gene: 13430 Mouse
    • Entrez Gene: 25751 Rat
    • Omim: 602378 Human
    • SwissProt: P50570 Human
    • SwissProt: P39054 Mouse
    • SwissProt: P39052 Rat
    • Unigene: 211463 Human
    • Unigene: 433257 Mouse
    • Unigene: 11231 Rat
    see all
  • 别名

    • CMT2M antibody
    • CMTDI1 antibody
    • CMTDIB antibody
    • DI CMTB antibody
    • Dnm2 antibody
    • DYN II antibody
    • DYN2 antibody
    • DYN2_HUMAN antibody
    • Dynamin II antibody
    • Dynamin-2 antibody
    • Dynamin2 antibody
    • DynaminII antibody
    • DYNII antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (ab3457)
    Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (ab3457)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y cells labeling Dynamin 2 with ab3457 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/2000 dilution (green). The nuclear counterstain is DAPI (blue), and F-actin is stained with Rhodamine Phalloidin iat 1/300 dilution (red). Negative control was cells without primary antibody present.

  • Western blot - Anti-Dynamin 2 antibody (ab3457)
    Western blot - Anti-Dynamin 2 antibody (ab3457)
    All lanes : Anti-Dynamin 2 antibody (ab3457)

    Lane 1 : SH-SY5Y whole cell lysate
    Lane 2 : PC-12
    Lane 3 : Neuro-2a
    Lane 4 : SK-N-AS
    Lane 5 : U- 87 MG
    Lane 6 : Rat Brain

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugated
  • Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (ab3457)
    Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (ab3457)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HeLa cells labeling Dynamin 2 with ab3457 at 2 ug/ml followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/2000 dilution (green).

    The nuclear counterstain is DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 2 antibody (ab3457)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 2 antibody (ab3457)
    ab3457 (1µg/ml) staining Dynamin 2 in human lung using an automated system (DAKO Autostainer Plus).
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (ab3457)
    Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 2 antibody (ab3457)
    ICC/IF image of ab3457 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3457, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (40)

发表研究结果有使用 ab3457?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3457 被引用在 40 文献中.

  • Ghosh M  et al. CD13 is a critical regulator of cell-cell fusion in osteoclastogenesis. Sci Rep 11:10736 (2021). PubMed: 34031489
  • Tian M  et al. The Expression of Dynamin 1, 2, and 3 in Human Hepatocellular Carcinoma and Patient Prognosis. Med Sci Monit 26:e923359 (2020). PubMed: 32573516
  • Ren N  et al. Dynamin 2 Is Correlated with Recurrence and Poor Prognosis of Papillary Thyroid Cancer. Med Sci Monit 26:e924590 (2020). PubMed: 32827429
  • Liu Y  et al. Arginine methylation of SHANK2 by PRMT7 promotes human breast cancer metastasis through activating endosomal FAK signalling. Elife 9:N/A (2020). PubMed: 32844749
  • Sajed R  et al. Overexpression and translocation of dynamin 2 promotes tumor aggressiveness in breast carcinomas. EXCLI J 19:1423-1435 (2020). PubMed: 33250680
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-6 of 6 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Dynamin 2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (neuron)
Permeabilization
Yes - 0.2%Triton
Specification
neuron
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 13 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Dynamin 2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (hippocampal neuron)
Permeabilization
Yes - 0.2%Triton
Specification
hippocampal neuron
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 13 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Dynamin 2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS-7)
Permeabilization
Yes - 0.2%Triton
Specification
COS-7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Ya Zhou

Verified customer

提交于 Oct 06 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Dynamin 2 antibody

Good
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary alveolar type II epithelial cells)
Specification
Primary alveolar type II epithelial cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% saponin
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 11 2012

Immunoprecipitation abreview for Anti-Dynamin 2 antibody

Excellent
Abreviews
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (lymphoblasts)
Total protein in input
5000 µg
Specification
lymphoblasts
Immuno-precipitation step
Protein A/G
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 07 2006

Question

Your data sheet indicated this antibody was tested only for western blotting. Do you have any information at all for immunoprecipitations of dynamin-2?

Read More

Abcam community

Verified customer

Asked on Nov 12 2003

Answer

Thank you for your enquiry. To our knowledge, Ab3457 has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any more questions, please contact us again.

Read More

Abcam Scientific Support

回复于 Nov 13 2003

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2023 艾博抗(上海)贸易有限公司版权所有