Dynamin 1重组抗体[EP801Y]_Dynamin 1抗体(ab52611)| Abcam中文官网

Product price, shipping and contact information

Currently unavailable

抱歉，我们目前无法显示此内容。

请联系我们以完成下单，或者之后再尝试访问。

正在加载规格和价格。。。

Abpromise

产品质量保证，客户专家支持，

点击获取更多

Western blot - Anti-Dynamin 1 antibody [EP801Y] (ab52611)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP801Y] to Dynamin 1
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free HRP

产品名称

Anti-Dynamin 1抗体[EP801Y]
参阅全部 Dynamin 1 一抗
描述

兔单克隆抗体[EP801Y] to Dynamin 1
宿主

Rabbit
经测试应用

适用于: WB, IHC-Pmore details
不适用于: ICC/IF
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide within Human Dynamin 1 aa 800-900 (C terminal). The exact sequence is proprietary.
阳性对照
- U-2 OS and 3T3 cell lysate, human brain tissue.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EP801Y
同种型

IgG
研究领域
- Neuroscience
- Processes

Alternative Versions
- HRP Anti-Dynamin 1 antibody [EP801Y] (ab207511)
- Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise™承诺保证使用ab52611于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		1/2000. Predicted molecular weight: 97 kDa.
IHC-P		1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

说明
WB 1/2000. Predicted molecular weight: 97 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

应用说明

Is unsuitable for ICC/IF.

功能

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis.
序列相似性

Belongs to the dynamin family.
Contains 1 GED domain.
Contains 1 PH domain.
细胞定位

Cytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated.
Target information above from: UniProt accession Q05193 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 1759 Human
- Entrez Gene: 13429 Mouse
- Entrez Gene: 140694 Rat
- Omim: 602377 Human
- SwissProt: Q05193 Human
- SwissProt: P39053 Mouse
- SwissProt: P21575 Rat
- Unigene: 522413 Human
- Unigene: 44736 Mouse
- Unigene: 10354 Rat
see all
别名
- B dynamin antibody
- D100 antibody
- DNM 1 antibody
- DNM antibody
- DNM1 antibody
- DYN1_HUMAN antibody
- Dynamin antibody
- Dynamin-1 antibody
- Dynamin1 antibody
see all

Western blot - Anti-Dynamin 1 antibody [EP801Y] (ab52611)

All lanes : Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/1000 dilution

Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : DNM1 knockout U-2 OS cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 97 kDa

ab52611 was shown to react with DNM1 in wild-type U-2 OS cells in Western blot with loss of signal observed in a DNM1 knockout cell line. Wild-type U-2 OS and DNM1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab52611 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] (ab52611)

Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Dynamin 1 antibody [EP801Y] (ab52611)

All lanes : Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/2000 dilution (purified)

Lane 1 : C6 cell lysate
Lane 2 : NIH/3T3 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 97 kDa
Observed band size: 97 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Dynamin 1 antibody [EP801Y] (ab52611)

Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/10000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 97 kDa
Observed band size: 97 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] (ab52611)

Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Dynamin 1 antibody [EP801Y] (ab52611)

Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/2000 dilution (unpurified) + 3T3 cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP antibody at 1/2000 dilution

Predicted band size: 97 kDa
Observed band size: 97 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] (ab52611)

Unpurified ab52611 at 1/100-1/250 dilution staining human brain tissue; paraffin embedded.
Anti-Dynamin 1 antibody [EP801Y] (ab52611)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab52611？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab52611 被引用在 14 文献中.

Sanghvi S et al. Inhibition of BKCa channels protects neonatal hearts against myocardial ischemia and reperfusion injury. Cell Death Discov 8:175 (2022). PubMed: 35393410
Kallergi E et al. Dendritic autophagy degrades postsynaptic proteins and is required for long-term synaptic depression in mice. Nat Commun 13:680 (2022). PubMed: 35115539
Tian M et al. The Expression of Dynamin 1, 2, and 3 in Human Hepatocellular Carcinoma and Patient Prognosis. Med Sci Monit 26:e923359 (2020). PubMed: 32573516
Dominguez-Meijide A et al. Effects of pharmacological modulators of a-synuclein and tau aggregation and internalization. Sci Rep 10:12827 (2020). PubMed: 32732936
Ackermann F et al. Critical role for Piccolo in synaptic vesicle retrieval. Elife 8:N/A (2019). PubMed: 31074746

View all Publications for this product

客户评价及客户问答

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Isotype control

应用

The Abpromise guarantee

靶标

功能

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (14)

客户评价及客户问答