DyLight® 650 Anti-Myc tag抗体[9E10] (ab117487)
Key features and details
- DyLight® 650 Mouse monoclonal [9E10] to Myc tag
- Suitable for: ICC/IF, Flow Cyt
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
DyLight® 650 Anti-Myc tag抗体[9E10]
参阅全部 Myc tag 一抗 -
描述
DyLight® 650小鼠单克隆抗体[9E10] to Myc tag -
宿主
Mouse -
偶联物
DyLight® 650. Ex: 654nm, Em: 673nm -
特异性
This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. -
经测试应用
适用于: ICC/IF, Flow Cytmore details -
免疫原
Synthetic peptide corresponding to Human Myc tag aa 400 to the C-terminus.
Database link: P01106 -
表位
Epitope located at aa 410-419; EQKLISEEDL -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1.4% Sodium phosphate, 0.88% Sodium chloride, 1% BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
9E10 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
- DyLight® 488 Anti-Myc tag antibody [9E10] (ab117499)
- DyLight® 550 Anti-Myc tag antibody [9E10] (ab117511)
- FITC Anti-Myc tag antibody [9E10] (ab117599)
- Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139)
- Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (ab202008)
- Anti-Myc tag antibody [9E10] (ab206486)
- Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)
- Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
- APC Anti-Myc tag antibody [9E10] (ab223896)
- Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
- Anti-Myc tag antibody [9E10] (ab32)
- HRP Anti-Myc tag antibody [9E10] (ab62928)
- PE Anti-Myc tag antibody [9E10] (ab72468)
- SureLight® APC Anti-Myc tag antibody [9E10] (ab72580)
- Biotin Anti-Myc tag antibody [9E10] (ab81658)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117487于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/500 - 1/5000.
|
|
Flow Cyt |
Use at an assay dependent concentration.
|
说明 |
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ICC/IF
1/500 - 1/5000. |
Flow Cyt
Use at an assay dependent concentration. |
靶标
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相关性
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
细胞定位
Nuclear -
别名
- c-myc tag antibody
- Myc Epitope Tag antibody
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab117487 被引用在 4 文献中.
- Li J et al. A universal reagent for detection of emerging diseases using bioengineered multifunctional yeast nanofragments. Nat Nanotechnol 18:1222-1229 (2023). PubMed: 37291255
- Shi G et al. Inhibition of neutrophil extracellular trap formation ameliorates neuroinflammation and neuronal apoptosis via STING-dependent IRE1α/ASK1/JNK signaling pathway in mice with traumatic brain injury. J Neuroinflammation 20:222 (2023). PubMed: 37777772
- Gupta R et al. Nov/CCN3 Enhances Cord Blood Engraftment by Rapidly Recruiting Latent Human Stem Cell Activity. Cell Stem Cell 26:527-541.e8 (2020). PubMed: 32197066
- McConnell AD et al. High affinity humanized antibodies without making hybridomas; immunization paired with mammalian cell display and in vitro somatic hypermutation. PLoS One 7:e49458 (2012). Flow Cyt ; Human . PubMed: 23166676