DyLight® 650 Anti-HA tag抗体[16B12] (ab117515)
![Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (ab117515)](https://www.abcam.cn/ps/products/117/ab117515/Images/ab117515-248151-anti-ha-tag-antibody-16b12-dylight-650-flow-cytometry.jpg "Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (ab117515)")
Key features and details
- DyLight® 650 Mouse monoclonal [16B12] to HA tag
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Species independent
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Isotype: IgG1
概述
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产品名称
DyLight® 650 Anti-HA tag抗体[16B12]
参阅全部 HA tag 一抗 -
描述
DyLight® 650小鼠单克隆抗体[16B12] to HA tag -
宿主
Mouse -
偶联物
DyLight® 650. Ex: 654nm, Em: 673nm -
特异性
ab117515 recognizes the Influenza hemagglutinin epitope (YPYDVPDYA). -
经测试应用
适用于: ICC/IF, Flow Cytmore details -
种属反应性
与反应: Species independent -
免疫原
-
常规说明
Conjugated to Dylight® 650 (ratio 1.3:): Visible absorption maxima 652 nm; Emission maximum 672 nm.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months. -
存储溶液
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.58% Sodium chloride, 95% Sodium phosphate -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
16B12 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117515于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500 - 1/5000.
|
|
| Flow Cyt | (1) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
1/500 - 1/5000. |
|
Flow Cyt
Use at an assay dependent concentration. |
靶标
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相关性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
别名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
图片
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Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (ab117515)This image is courtesy of an anonymous Abreview
ab117515 staining 293T cells expressing a HA-tagged protein (intracellular) by Flow Cytometry. Cells were permeabilized and fixed. The sample was incubated with the primary antibody (1/200) for 30 minutes at 4°C.
Gating Strategy: Gated on FSC-SSC to exclude debris/dead cells.
Grey - unstained cells.
Red - Wild type 293T, stained.
Blue - 293T expressing HA-tagged protein, stained.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab117515 被引用在 7 文献中.
- Jacquot P et al. Engineering of a Bispecific Nanofitin with Immune Checkpoint Inhibitory Activity Conditioned by the Cross-Arm Binding to EGFR and PDL1. Biomolecules 13:N/A (2023). PubMed: 37189383
- Lucken-Ardjomande Häsler S et al. GRAF2, WDR44, and MICAL1 mediate Rab8/10/11-dependent export of E-cadherin, MMP14, and CFTR ?F508. J Cell Biol 219:N/A (2020). PubMed: 32344433
- Nafria M et al. Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells. Cell Rep 31:107691 (2020). PubMed: 32460028
- Gabaev I et al. Quantitative Proteomics Analysis of Lytic KSHV Infection in Human Endothelial Cells Reveals Targets of Viral Immune Modulation. Cell Rep 33:108249 (2020). PubMed: 33053346
- Marelli S et al. Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest. Elife 9:N/A (2020). PubMed: 32292164
- Feng Y et al. LRRC25 Functions as an Inhibitor of NF-?B Signaling Pathway by Promoting p65/RelA for Autophagic Degradation. Sci Rep 7:13448 (2017). PubMed: 29044191
- Greenwood EJ et al. Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. Elife 5:N/A (2016). PubMed: 27690223